中国小型猪内源性反转录病毒研究进展与对策  

Research Progress and Countermeasures of Porcine Endogenous Retrovirus in Chinese Miniature

作　　者：冯书堂[1,2] 马玉媛[3] 夏颖[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]北京盖兰德生物科技有限公司,北京100007 [3]军事医学科学院输血医学研究所,北京100850

出　　处：《中国畜牧兽医》2017年第4期1244-1248,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家"863"(2012AA020603);国家转基因专项(2008ZX08012-002-05);北京市科技成果转化项目(2015-02)

摘　　要：作者介绍了国内外猪内源性反转录病毒(PERV)研究进展,简述了中国不同品种小型猪PERV存在情况、PERV前病毒全基因克隆与序列分析、细胞水平鉴定方法、感染HEK293细胞研究平台的创建、猪A3F对PERV的抑制作用研究,以及从分子遗传学上揭示不同品系PERV特异性等创新性研究成果,分析了五指山小型猪近交系具有PERV基因拷贝少且没有PERV-C等特异性,以及展望培育中国PERV阴性猪新品系方法等研究,以期为人类异种器官移植和生物医用材料产品安全性研发,解决小型猪PERV疾病传播危险性提供科学对策和新的方向。The paper is a brief introduction of porcine endogenous retrovirus （PERV） innovative research results in the world and also expounds the passing PERV existence situation on different varieties of miniature pig, analyzes the PERV-virus gene cloning and sequence, appraises method on cell level, create the platform of infection HEK293 cells research , study on pigs A3F inhibition of PERV, and reveal the innovative research results on the specific molecular genetics in the different strain of PERV, analyzes the advantages of Wuzhishan miniature pig inbred line such as low gene copy of PERV, and there is no passing PERV-C specificity and as well as looking forward to cultivate the methods for new strain of PERV negative pigs. It will provide a scientific counter measure and new perspective to solve the spread of disease risk of miniature pig PERV and product safety for human xenotransplantation and biomedical materials of research and development.

关 键 词：中国小型猪 猪内源性反转录病毒 感染性克隆 异种移植 

分 类 号：S852.659.3[农业科学—基础兽医学]