丝素降解生成的低分子多肽对细胞生长性能的影响  

Effect of low-molecular-weight silk fibroin peptides on cell growth property

作　　者：王富平[1] 庞亚妮[1] 陈忠敏[1] 

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《丝绸》2017年第4期1-4,共4页Journal of Silk

基　　金：重庆市科委(csct2014jcyja10018);重庆市教委(KJ1400916)

摘　　要：分析丝素降解生成的低分子多肽(low-molecular-weight silk fibroin peptides,LMW-SF)对细胞生长的影响。LMW-SF通过中性蛋白酶酶解丝素蛋白(silk fibroin,SF)获得,分别采用过滤除菌和高压蒸汽灭菌处理后,在0.1~5.0 mg/m L体积浓度条件下,检测LMW-SF对人正常肝细胞(L02)、人肝癌细胞(HepG2)的生长影响,计算相对增殖率(relative growth rate,RGR)。结果显示:当LMW-SF体积浓度较低(0.1 mg/m L和0.5 mg/m L)时,LMW-SF对细胞生长影响较小;当其体积浓度较高(2.5 mg/m L和5.0 mg/m L)时,RGR值最低至8.3%,呈强抑制细胞生长作用;高压蒸汽灭菌处理后,RGR最高达227.4%,呈强促细胞生长作用。研究结果表明:高压蒸汽处理可以使LMW-SF变性失活,并实现强促细胞生长作用,可成为蛋白类物质应用于组织工程支架时的必需工艺环节。The main purpose of this paper is to analyze the effects of low-molecular-weight silk fibroin peptides（LMW-SF） on cell growth characteristics.Firstly,LMW-SF was achieved from silk fibroin enzymolysis by neutral protease.Secondly,LMW-SF was sterilized by filtration and high-pressure steam,respectively.Finally,the effects of LMW-SF on the growth of human normal liver cell（L02） and human liver cancer cell（HepG2） were tested under the volume concentration of 0.1 - 5.0 mg/mL,and the relative growth rate（RGR） was calculated.The results show that LMW-SF have slight effect on cell growth at low concentration（0.1 mg/mL and 0.5 mg/mL）; when the volume concentration is high（2.5 mg/mL and 5.0 mg/mL）,the lowest value of RGR is 8.3%,indicating a strong inhibition effect on cell growth.RGR is up to 227.4% after high-pressure steam sterilization,showing a strong promotion effect on cell growth.It is obvious that LMW-SF have a strong effect on promoting cell growth after highpressure steam sterilization,and high-pressure steam sterilization could be a necessary process when protein is applied in tissue engineering scaffolds.

关 键 词：低分子丝素蛋白多肽 酶解 过滤除菌 高压蒸汽灭菌 细胞生长 

分 类 号：R318.08[医药卫生—生物医学工程]