蟾皮华蟾毒配基组分体外对肿瘤及正常细胞株的抑制作用  

Inhibitory effect of cinobufagin fraction of the skin of Bufo bufo gargarizans cantor on tumor and normal cell lines in vitro

作　　者：陈贵兵[1] 李荣[2] 夏绍友[2] 

机构地区：[1]成都军区总医院普外中心,成都610083 [2]解放军总医院普通外科,北京100853

出　　处：《天津中医药大学学报》2017年第2期128-131,共4页Journal of Tianjin University of Traditional Chinese Medicine

摘　　要：[目的]测定蟾皮第18组分(F18)的化学成分并考察其体外对不同细胞株的抑制作用。[方法]通过质谱法对已分离的F18进行测定,采用噻唑蓝法(MTT法)检测F18对人SW480、SW620、BGC823肿瘤细胞株、人L02、CIK正常细胞株及鼠C26、CT26、4T1肿瘤细胞株的抑制作用,CCK-8法检测F18对SW620、L02、CIK在不同时间点的细胞存活率,最后流式细胞术检测F18对SW620细胞周期的影响。[结果]经鉴定蟾皮F18的主要成分为华蟾毒配基,故将蟾皮F18命名蟾皮华蟾毒配基组分(CFSB)。CFSB对SW480、SW620、BGC823、L02、CIK、C26、CT26、4T1细胞的IC_(50)值分别为1.10、1.15、1.55、4.85、2.63、21.22、31.39、20.75 mg/L,对SW620、L02、CIK细胞存活率呈时间依赖性。流式细胞术测定浓度在2 mg/L的CFSB干预SW620细胞24 h后其S期、G_2/M期细胞比例分别由36.41%增至55.73%、10.89%增至16.95%。[结论]CFSB对肿瘤细胞和正常细胞的增殖均有抑制作用,可使SW620细胞周期阻滞在S期和G_2/M期。[Objective] To detect chemical composition of the eighteenth fraction（F18）of toad skin and observe its inhibitory effects on tumor and normal cell lines in vitro. [Methods] First, to test separated F18 by mass spectrometry（MS）. Then, to test inhibitory effects of different cell lines（SW480, SW620, BGC823, L02, CIK, C26,CT26, 4T1）with F18 interfered by the MTT method, and to detect the survival rate of SW620, L02, CIK with F18 interfered by CCK-8 method in different time. Last, to detect variation of cell cycle of SW620 with F18 interfered by flow cytometry. [Results] The main component of F18 was cinbufagin, so the F18 was named Cinobufagin Fraction of the Skin of Bufo bufo gargarizans cantor（CFSB）. Half-maximal inhibitory concentration values of eight cell lines（SW480、SW620、BGC823、L02、CIK、C26、CT26、4T1）were 1.10、1.15、1.55、4.85、2.63、21.22、31.39、20.75 μg/mL,respectively. The survival rate of SW620, L02 and CIK showed time dependence. The proportion of S and G_2/M phase of SW620 cell which was interfered in 2 μg/mL CFSB for 24 h increased from 36.41% to 55.73% and 10.89%to 16.95%, respectively. [Conclusion] CFSB can inhibit the proliferation of tumor and normal cells and also can induce S and G_2/M phases cell-cycle arrest in SW620 cell.

关 键 词：蟾皮 华蟾毒配基 中药组分 质谱 肿瘤 

分 类 号：R285.5[医药卫生—中药学]