蔬菜中多种有机磷农药残留的固相萃取-气相色谱测定方法研究  

Simultaneous determination of organophosphorus pesticide residues in vegetables by solid phase extraction coupled with gas chromatography (SPE-GC)

作　　者：黄芬[1] 夏文斌[1] 李雄伟[1] 欧桂香[1] 周瑞芳[1] HUANG Fen XIA Wen-bin LI Xiong-wei OU Gui-xiang ZHOU Rui-fang(Ningxiang County Center for Disease Control and Prevention, Ningxiang, ttunan 410600, China)

机构地区：[1]宁乡县疾病预防控制中心,湖南宁乡410600

出　　处：《实用预防医学》2017年第5期627-629,共3页Practical Preventive Medicine

摘　　要：目的建立同时测定蔬菜中多种常见有机磷农药残留的固相萃取-气相色谱法。方法样品经乙腈提取、固相小柱净化,气相色谱法进行测定。结果在优化的条件下,多达19种的有机磷农药均能较好的分离,峰面积与浓度之间呈现良好线性,相关系数r≥0.9994,定量检出限为2.5~16.9μg/kg。蔬菜加标回收率为62.4%~111.5%,相对标准偏差(RSD)为0.74%~5.26%。结论该法操作简便,灵敏,准确,重现性好,适用于蔬菜中多种常见有机磷农药残留的测定和分析。Objective To establish a method for the simultaneous determination of common organic phosphorus pesticide residues in vegetables by solid phase extraction coupled with gas chromatography （ SPE- GC）. Methods Samples were extracted by acetonitrile, cleaned up on a solid phase column purification and determined by SPE-GC. Results Under the selected con- ditions, up to 19 kinds of organic phosphorus pesticide could be completely separated and showed good linearity. The correlation coefficient （r） was ≥ 0.9994, with the detection limit of 2.5-16.9 μg/kg. The recovery rate of standard addition was 62.4%- 111.5%, and the relative standard deviation （ RSD ） was 0.74%-5.26%. Conclusions The experimental results show that the method is simple, highly sensitive and accurate, with a good reproducibility. It is suitable for the determination and analysis of the common organophosphorus pesticide residues in vegetables.

关 键 词：有机磷农药 气相色谱法 固相萃取 蔬菜 

分 类 号：R155.5[医药卫生—营养与食品卫生学]