褪黑素与乳腺癌大鼠外周血Treg相关细胞因子的关系研究  

Study on Relationship between Melatonin and Treg Related Cytokines in Peripheral Blood of Rats with Breast Cancer

作　　者：黄玉钿[1] 郑曦[1] 薛玉钦 吴进[1] 

机构地区：[1]福建医科大学附属福州市第一医院,福建福州350009

出　　处：《中国医学创新》2017年第11期28-30,共3页Medical Innovation of China

基　　金：福建省自然科学基金(2015J01503)

摘　　要：目的:研究褪黑素与乳腺癌大鼠外周血血清中转化生长因子β1(Transforming growth factor-β1,TGF-β1)和白介素35(Interleukin-35,IL-35)水平的关系,分析褪黑素对乳腺癌大鼠外周血中调节性T细胞(Regulatory T cell,Treg cell)相关细胞因子及肿瘤免疫的影响。方法:选用两月龄雌性SD大鼠60只,接种大鼠乳腺癌SHZ-88细胞,按照随机数字表法分成实验组(注射褪黑素)和对照组(注射生理盐水),10 d后乳腺癌大鼠荷瘤动物模型建成,抽取大鼠股动脉外周血,采用ELISA法检测外周血血清中TGF-β1、IL-35的浓度。结果:实验组乳腺癌大鼠外周血TGF-β1浓度低于对照组,差异有统计学意义(P<0.05);实验组乳腺癌大鼠外周血IL-35浓度低于对照组,差异有统计学意义(P<0.05)。结论:褪黑素能降低乳腺癌大鼠外周血中Treg相关细胞因子的表达,褪黑素可能具有抑制乳腺癌细胞免疫逃避从而拮抗乳腺癌进展的作用。Objective： To study the relationship between melatonin and expression levels of transforming growth factor- β 1 and interieukin-35 in peripheral blood serum of breast cancer rats, and to analyze the effect of melatonin on regulatory T cell related cytokines in peripheral blood and tumor immunity of breast cancer rats. Method： A total of 60 female SD rats in age 2 months were selected, and the rat breast cancer SHZ-88 cells were inoculated in the rats.The rats were randomly divided to the experimental group injected with Melatonin and the control group injected with saline.The tumor bearing animal models of breast cancer rats were established on the 10th day after inoculating.The peripheral blood of rats femoral artery, and the concentrations of TGF- β1 and IL-35 in peripheral blood serum were detected by ELISA method.Result： In the experimental group, the concentration of TGF- β1 in peripheral blood of breast cancer rats was lower than that of the control group （ P〈0.05 ） .In the experimental group, the concentration of IL-35 in peripheral blood of breast cancer rats was lower than that of the control group （ P〈0.05 ） .Conclusion： Melatonin can reduce the expression of Treg related cytokines in the peripheral blood of breast cancer rats, which may have the effect of inhibiting the immune evasion of breast cancer cells to antagonize the progression of breast cancer.

关 键 词：褪黑素 乳腺癌 TREG细胞 转化生长因子Β1 白介素35 

分 类 号：R737.9[医药卫生—肿瘤]