基于量子点/抗体探针采用电化学方法检测水中微囊藻毒素  

Determination of Microcystin in Water Using Electrochemical Method Based on Quantum Dot/Antibody Probe

作　　者：李莹[1] 孙秀兰[1] 张银志[2] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]食品科学与技术国家重点实验室江南大学,江苏无锡214122

出　　处：《食品与生物技术学报》2017年第2期136-142,共7页Journal of Food Science and Biotechnology

基　　金：国家973计划项目(2012CB720804);国家自然科学基金委员会-广东省人民政府自然科学联合基金项目(U1301214);2010年度公益性行业(农业)科研专项(201003008-08)

摘　　要：用量子点偶联微囊藻毒素抗体作为探针,经过间接竞争反应之后,通过方波溶出伏安法测定Cd^(2+)的浓度,从而实现对水中微囊藻毒素含量的检测。分别对缓冲液p H、浓度、沉积电位、沉积时间等因素进行优化。实验结果表明:Cd^(2+)在-0.768 V左右会出现一个灵敏的溶出峰,在最优实验条件下进行检测,峰电流值与微囊藻毒素浓度的对数值在一定范围内呈良好的线性关系,线性范围在0.1~8μg/L之间,线性方程为y=-196.66x+242.15,相关系数R2=0.992 45,检出限为0.08μg/L,加标回收率为91.0%~108.6%,相对标准偏差为2.97%~6.98%。With the probe of quantum dots coupled with microcystin antibody （QDs-Ab）, the content of microcystin was determined by measuring cadmium ions released from QDs based on square wave stripping voltammetry after indirect competitive reaction. The electrochemical response was optimized with respect to pH, buffer concentration, deposition potential, deposition time and so on. Results showed that the sensitive stripping peak potential of cadmium ions was about -0.768 V. Under the optimal conditions, the peak current was linear with the logarithm of microcystin concentration in the range of 0.1 -8 μg/L and the equation of regression was 7=-196.66x＋242.15 （ R2 =0.994 8） with a detection limit of 0.08×109. The recovery of standard addition experiment was in the range of 91 %-108.6% with the relative standard deviation of 2.97 %-6.98%.

关 键 词：微囊藻毒素 量子点 镉离子 方波溶出伏安法 

分 类 号：X832[环境科学与工程—环境工程]