海洋细菌Planococcus rifietoensis发酵产色素培养基的优化  

Optimization of Fermentation Medium for the Production of Pigments by Marine Bacteria Planococcus rifietoensis

作　　者：孔菲[1,2,3] 蔡宇杰[2] 许晨[3] 许建中[3] 夏金梅[3] 

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [3]国家海洋局第三海洋研究所海洋生物遗传资源重点实验室,福建厦门361005

出　　处：《食品与生物技术学报》2017年第2期179-186,共8页Journal of Food Science and Biotechnology

基　　金：中央级公益性科研院所基本科研业务费专项资金资助项目(海三科2015027)

摘　　要：在单因素的基础上,以Plackett-Burman(PB)设计结合响应面(RSM)分析法,对产色素海洋细菌Planococcus rifietoensis发酵培养基的8个营养成分配比进行优化。结果表明:培养基最佳碳氮源分别是葡萄糖和蛋白胨,葡萄糖、MgSO_4·7H_2O和酵母粉添加量显著影响色素的产量。Box-Benhnken设计分析确定最优培养基配方为:葡萄糖9.78 g/L,蛋白胨8.00 g/L,酵母粉2.05g/L,MgSO_4·7H_2O 8.17 g/L,Na_2HPO_40.1 g/L,FeSO_4·7H_2O 0.005 g/L,NaCl 10 g/L,CaCl_2 0.1 g/L,培养54 h,在此培养条件下,色素的OD值可达0.984,比优化前提高了245%。Based on monofactorial experiments,Plackett-Burman（PB）design and response surface methodology（RSM）were applied to optimize 8 medium components for the production of pigments by marine bacteria strain Planococcus rifietoensis.Results showed that optimal carbon and nitrogen sources of the medium were glucose and peptone,respectively,and glucose,yeast extract and MgSO_4·7H_2O had major effects on the production of pigments.The optimal culture medium was comprised of glucose 9.78 g/L,peptone 8.00 g/L,yeast extract 2.05 g/L,MgSO_4·7H_2O 8.17 g/L,Na_2HPO_4 0.1 g/L,FeSO_4·7H_2O 0.005 g/L,NaCl 10 g/L and CaCl_2 0.1 g/L.The incubation time was54 hours.Under these conditions,the optical density（OD）of pigments reached to 0.984,which was 245% higher than that before optimization.

关 键 词：海洋细菌 响应面优化 色素 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TS264.4