缺血后适应对大鼠大脑缺血再灌注脑损伤的S-100B蛋白、超敏感性C反应蛋白表达的影响  

Effects of ischemic injury on the expression of S-100B protein and ultra sensitive C reactive protein in cerebral ischemia reperfusion injury in rats

作　　者：林先刚[1] 李兴亚[1] 唐巍[1] 陈业农[1] 

机构地区：[1]安徽中医药大学针灸骨伤临床学院,合肥230038

出　　处：《长春中医药大学学报》2017年第2期188-191,共4页Journal of Changchun University of Chinese Medicine

基　　金：安徽省教育厅自然科学基金重点项目"基于RISK-PI3K信号通路研究脑络欣通干预局灶性脑缺血后适应的作用机制"(KJ2015A131);安徽中医学院自然科学基金项目"基于缺血后适应的益气活血中药干预再灌注大鼠脑损伤机制研究"(2013zr003);安徽中医药大学自然科学基金项目"姜黄素对香烟诱导COPD大鼠免疫失衡的影响"(2014qn002)

摘　　要：目的探讨缺血后适应对脑缺血大鼠再灌注损伤(IRI)的S-100B蛋白、超敏感性C反应蛋白含量表达的影响,比较在不同时间位点的作用特点。方法采用线栓法制作大鼠动脉缺血再灌注动物模型。Wistar大鼠随机分为6组:假手术组(Sham组),IRI组,缺血后适应(IP)Ⅰ~Ⅳ组。检测脑损伤标志物S-100B蛋白、超敏感性C反应蛋白(h-CRP)含量变化。结果与IRI组比较,IPⅠ~Ⅳ各组大鼠神经功能缺损评分、S-100B及C反应蛋白(h-CRP)显著降低,且IPⅠ~Ⅳ各组依次递增,数据均有统计学意义。结论缺血后适应可改善大鼠术后神经功能缺损评分,下调S-100B蛋白、超敏感性C反应蛋白含量表达,对局灶性脑缺血再灌注损伤大鼠具有保护作用。Objective To investigate adaptation ischemic cerebral ischemia- eperfusion injury （ischemia reperfusion injury, IRI） of S-100B protein, ultra- sensitivity C-reactive protein expression comparison sites in different time characteristics. Methods Using suture method cerebral artery occlusion and reperfusion animal model. Wistar rats were randomly divided into six groups： sham operation group （Sham group）, IRI group, ischemic postconditioning （ischemia postconditioning, IP） I - IV group. Content change of S- 100B protein and super sensitive C reactive protein （h-CRP） in brain injury markers. Results Compared with the IRI group, the neurological deficits score, S-100B and C reactive protein （h-CRP） were significantly decreased in all groups, and the IP I-IV groups were increased in turn. The data were statistically significant. Conclusion After ischemia adaptation can improve the postoperative rats neural function defect score and down regulation of S-100B protein, hypersensitive C reaction protein expression, after focal cerebral ischemia reperfusion injury in rats has a protective effect.

关 键 词：缺血再灌注损伤 S-100B蛋白 超敏感性C反应蛋白 

分 类 号：R364.4[医药卫生—病理学]