microRNA-10b调控SW480结肠癌干细胞生物学行为的研究  

Study on the biological behavior of SW480 cancer stem cells regulated by microRNA-10b gene

作　　者：杨国华[1] 郑圣斌 施梅英[1] 黄若磊[1] 

机构地区：[1]福建医科大学省立临床医学院福建省立医院南院普外科,福州350007

出　　处：《福建医药杂志》2017年第2期48-50,共3页Fujian Medical Journal

摘　　要：目的探讨microRNA-10b对SW480结肠癌干细胞迁移及侵袭功能影响。方法流式细胞仪分选SW480结肠癌干细胞,通过转染技术抑制SW480结肠癌干细胞microRNA-10b表达,划痕愈合实验观察癌干细胞迁移能力变化,Transwell法观察癌干细胞侵袭能力变化。结果抑制SW480结肠癌干细胞microRNA-10b表达,癌干细胞转染48h后,其迁移率(45.4±3.1)%与转染前(86.8±12.4)%比较明显下降(P<0.05);转染后癌干细胞侵袭力也明显下降,转染前侵袭细胞数为(486.4±124.7)个,转染后为(297.5±48.0)个(P<0.05)。结论 microRNA-10b基因可调控SW480结肠癌干细胞迁移及侵袭功能,抑制其表达可降低SW480结肠癌干细胞迁移力及侵袭力。Objective To investigate the effect of microRNA-10b on the migration and invasion of SW480 cancer stem ceils. Methods Sorting SW480 cancer stem ceils by Flow cytometry, inhibiting SW480 cancer stem cell line through transfee tion, and observating the cancer stem cell migration ability changes by wound healing experiment, also observatlng invasion abili ty changes through Transwell experiment. Results Inhibiting the expression of microRNA-10b in SW480 cancer stem cell, after transfection 48 h later, the migration rate （45.4±3.1）% which was significantly lower than that of non-tranfeeted group （86.8 ± 12.4）% （P〈0.05）. cancer stem cell invasion force also decreased significantly through transfection, invasion cell number was （48.64±124.7） in the non- transfected group, and that was （297.5±4.8） in the transfected group, there was significantly difference between the two groups, P〈 0.05. Conclusion MicroRNA 10b gene can regulate the migration and invasion of SW480 cancer stem cells, and inhibiting its expression can reduce the migration and invasion of cancer stem cells.

关 键 词：microRNA-10b 结肠癌 癌干细胞 迁移 侵袭 

分 类 号：R735.35[医药卫生—肿瘤]