细胞周期蛋白依赖性激酶抑制剂LS-007在急性淋巴细胞白血病中作用及机制  

Effect of CDK Inhibitor LS-007 on Acute Lymphoblastic Leukemia and Its Mechanism

作　　者：吕江峰[1] 徐凤玲[2] 鹿军[3] 任玉国[1] 

机构地区：[1]莱芜市人民医院检验科,山东莱芜271100 [2]莱芜市人民医院输血科,山东莱芜271100 [3]莱芜市人民医院血液科,山东莱芜271100

出　　处：《中国实验血液学杂志》2017年第2期393-397,共5页Journal of Experimental Hematology

摘　　要：目的:研究细胞周期蛋白依赖性激酶(CDK)抑制剂LS-007在急性淋巴细胞白血病中的作用及其机制。方法:培养急性淋巴细胞白血病细胞株,以LS-007、夫拉平度、ABT-199等药物处理细胞,然后用mRNA定量和PCR免疫蛋白印迹方法检测白血病细胞内凋亡相关因子mRNA和蛋白含量的变化。用凋亡试剂盒检测细胞凋亡情况。结果:mRNA定量和免疫蛋白印迹检测结果表明,经LS-007处理的急性淋巴细胞白血病细胞中抗凋亡蛋白含量显著下降,LS-007和ABT-199联合使用可促进细胞凋亡,效果更佳。结论:LS-007可以影响RNA聚合酶位点磷酸化,从而改变细胞依赖性周期蛋白激酶,促进细胞凋亡,在急性淋巴细胞白血病的缓解中具有一定的积极意义。Objective： To study the effect of cyclin dependent kinase （CDK） inhibitor LS-007 on acute lymphoblastic leukemia and its mechanism. Methods： The acute lymphocytic leukemia cell line was cultured and treated by LS-007, flavopiridol and ABT-199, then the changes of apoptosis-related factor mRNA and protein levels were detected by using mRNA quantitative PCR and Werstern blot. Results： quantitative PCR and Western blot detection showed that the levels of antiapoptotic protein decreased significantly in acute lymphoblastic leukemia cells after LS-007 treatment, and the pro- apoptotic effect of LS-007 combined with ABT-199 was much better. Conclusion： LS-007 can affect the phosphorylation of RNA polymerase sites and promote cell apoptosis through changing the activities of CDK, thus having some positive significance for relieving acute lymphoblastic leukemia.

关 键 词：急性淋巴细胞白血病 细胞周期性蛋白激酶 LS-007 

分 类 号：R733.71[医药卫生—肿瘤]