从杂草DRB中筛选微生物除草剂的研究  

SCREENING MICROBIAL HERBICIDES FROM WEED DRB

作　　者：但汉斌[1] 陈永强 魏雪生[1] 朱珠[1] 唐柳[1] 李永泉[2] 庄晓峰[2] 

机构地区：[1]天津农科院园艺所农业微生物研究室,天津300384 [2]浙江大学生命科学学院,杭州310028

出　　处：《微生物学通报》2002年第4期5-9,共5页Microbiology China

基　　金：天津农业科学院院长科技基金资助项目(N0.97909);浙江省科技计划资助项目(No.981103084)

摘　　要：采用MPC和CVP富集培养基从马唐和狗尾草的根部分离到假单胞菌792株、欧文氏菌515株。通过对大肠杆菌的抗代谢毒性试验、对原宿主的萌发抑制试验、杀草试验以及对两种草坪草的安全性试验,筛选的S7菌株能100％抑制狗尾草的种子萌发,对供试草坪草不仅没有负影响,而且对高羊茅的种子萌发还有轻微的促生作用。尽管S7在狗尾草的芽后处理中校正防效只有56.7％,但分析认为以杂草DRB作微生物除草剂的目标菌株会更有前途。By using enrichment media NPC and CVP, 792 strains of Pseudomonas and 515 strains of Erwinia were isolated from the rhizosphere of Digitaria adscendens (H. B. K.) Hem and Setaria vindis (L.) Beanv. Following which, experiments of antimetabolic test with E. coli, seed emergence controlling of S. viridis, herbicidal activity and security with green grass were carried out to select the desired bacteria. As the result, the selected strain, S7, could wholly control the seed emergence of S. viridis without any harm to the two tested green grass. And more, S7 promoted the seed emergence of Festuca arundinacea slightly. In spite of the comparatively low corrected mortality (56. 7%) of S7 after emergence of S. viridis, Selecting of microbial herbicides from weed DRB is thought to be more prospective.

关 键 词：杂草 DRB 筛选 微生物除草剂 根际细菌 狗尾草 马唐 欧文氏菌 假单胞菌 

分 类 号：S476[农业科学—农业昆虫与害虫防治]