纤维素降解菌的筛选、鉴定和糖化水平研究  

Isolation,identification and saccharification level of cellulose degrading bacteria

作　　者：郑丽[1,2] 张海鹏[2] 宋艳培 刘孟浩 何时雨[1] 马旭东[1] 覃新导[4] 李伯松[1] 

机构地区：[1]中国热带农业科学院广州实验站,广东广州510140 [2]华南农业大学农学院,广东广州510642 [3]华南农业大学材料与能源学院,广东广州510642 [4]中国热带农业科学院,海南海口571101

出　　处：《广东农业科学》2017年第2期104-111,共8页Guangdong Agricultural Sciences

基　　金：中国热带农业科学院院本级基本科研业务费专项资金(1630052015047);海南省自然科学基金(314185);广东省科技计划项目(2015A020209027)

摘　　要：将前期从木薯生境中分离、保存的298个菌株,通过平板测试,筛选到57个可产纤维素酶活的菌株,其中酶活性效果最好的菌株(水解圈最大,将其赋值得分为3)所占比例为40.9%,酶活性依次减弱的菌株所占比例(赋值得分为2和1)分别占27.9%和24.6%。采用DNS法测定57个菌株的糖化水平和酶活力,结果显示,10株细菌具有较好潜力降解纤维素,其中菌株HWY-3-9的糖化和酶活力最好,产糖量为0.1852 mg/m L,酶活力为0.0012 U/m L,经鉴定为解淀粉芽孢杆菌。In this study, 298 bacteria were isolated from cassava microenvironments and 57 strains with cellulase activity were gained by in vitro detection test. Results showed that strains with big hydrolysis circle （the score of 3） accounted for 40.9% and strains with weakly hydrolysis circle （ the score of 2 and 1 ） accounted for 27.9% and 24.6%, respectively. The saccharification level and enzyme activity were tested by DNS method, and we found 10 potential strains with efficient ability of degradation of cellulose, in which strain HWY-3-9 had the best saccharification and enzyme activity, the sugar content was 0.1852 mg/mL and enzyme activity was 0.0012 U/mL, and it was identified as Bacillus amyloliquefaciens.

关 键 词：细菌 纤维素酶 糖化水平 

分 类 号：S182[农业科学—农业基础科学]