蕨麻猪与巴马小型猪脏器指数及低氧相关基因的比较  

Comparison of organ coefficients and expression of hypoxia-related genes in the Bama and Juema minipigs

作　　者：牛廷献[1] 王红义[1] 郭晓宇[1] 陆璐[1] 申健[1] 肖攀[1] 冯小明[1] 

机构地区：[1]兰州军区兰州总医院动物实验科,兰州730050

出　　处：《中国比较医学杂志》2017年第4期9-13,共5页Chinese Journal of Comparative Medicine

基　　金：甘肃省自然科学基金项目(1308RJZA178)

摘　　要：目的研究蕨麻猪与巴马小型猪脏器指数及低氧相关基因表达量的差异。方法采用Taq Man探针实时定量PCR(real-time quantitative PCR)技术,检测蕨麻猪与巴马小型猪心、肝、脾、肺、肾低氧反应基因表达量的变化。结果蕨麻猪的肾脏、脾脏指数低于巴马小型猪,蕨麻猪的心脏、肺脏指数高于巴马小型猪。蕨麻猪血管内皮细胞生长因子(Vascular endothelial growth factor,VEGF)与低氧诱导因子-1α(hypoxia inducible factor 1,HIF-1α)在肺、肾中的表达量明显比普通猪高(P<0.05或P<0.01);促红细胞生成素(erythropoietin,EPO)只在肺中比巴马小型猪高(P<0.05)。结论不同物种脏器指数可能是物种对物质环境、能量环境、外界病原体、能量代谢需求等综合因素进化适应的结果。蕨麻猪低氧相关基因明显比巴马小型猪高,揭示在基因方面蕨麻猪具有较强的高原适应性。Objective To compare the organ coefficients and expressions of hypoxia-related genes in Bama and Juema pigs. Method Real-time quantitative PCR was used to detect the changes of hypoxia gene expressions in the heart, liver, spleen, lung, and kidney of Juema and Bama miniature pigs. Results The organ coefficients of kidney and spleen of Juema pigs were significantly lower than Bama miniature pigs （P 〈 0.05 for both）. The heart and lung coefficients of Juema pigs were significantly higher than that of Bama miniature pigs （P 〈 0.05 for both）. The VEGF and HIF-la expressions in the lung and kidney in Juema pigs were significantly higher than Bama pigs （P 〈 0. 05 or P 〈 0. 01 ）. Only the EPO expression in in the lung of Juema pigs was significantly higher than that of the Bama miniature pigs （P 〈 0. 05 ）. Conclusions These results indicate that the variation in organ coefficients may be resulted from evolutionary factors such as adaptiveness to environmental physical and energy conditions, pathogens, and energy metabolism demands, etc. in combination. Juema miniature pigs showing a significantly higher expression of hypoxia-related genes than that in Bama minipigs indicate that it has a strong plateau adaptability by higher gene expressions.

关 键 词：蕨麻猪 巴马小型猪 脏器指数 低氧相关基因 比较 

分 类 号：R-33[医药卫生]