辣蓼总黄酮提取工艺及其抗菌活性  

Extraction processand antibacterial activity on total flavonoids from Polygonum hydropiper

作　　者：石跃武 刘启志 蒋红 王宏军 刘悦 

机构地区：[1]辽宁省凌海市石山镇动物卫生监督所,辽宁凌海121200 [2]辽宁省新民市畜牧技术推广站,辽宁新民110300 [3]锦州医科大学畜牧兽医学院,辽宁锦州121001

出　　处：《现代畜牧兽医》2017年第4期5-8,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：国家自然科学基金项目(31201951);辽宁省高等学校优秀人才支持计划项目(LJQ2013087);辽宁省自然科学基金(2014022051);辽宁省大学生创新创业训练计划项目(201410160049)

摘　　要：为了研究中药辣蓼中总黄酮的提取工艺及其抗菌活性。采用均匀设计试验法,以总黄酮含量为响应值,考察了乙醇浓度、料液比、提取时间、提取温度对其影响,用DPS数据处理系统软件进行结构分析;采用微管-平板法测定辣蓼提取物对金黄色葡萄球菌的抗菌活性。结果显示,辣蓼总黄酮的提取工艺条件为:料液比1:60,乙醇浓度70%,提取时间1 h,提取温度为50℃,在该条件下,辣蓼中总黄酮含量为5.35%±0.23%,辣蓼总黄酮对金黄色葡萄球菌的最小抑菌浓度为0.782 mg/mL。该提取工艺操作简便,可用于中药辣蓼中总黄酮的提取且提取物对金黄色葡萄球菌有抗菌活性。In order to study the extraction process of total flavonoids from Polygonum hydropiper in traditional Chinese medicine and its antibacterial activity, 4 factors included the ethanol concentration, solid-liquid ratio, extraction time, extraction temperature, were studied by the uniform design method and the content of total flavonoids were as the index. The extraction process parameters were analyzed by 9.5 version DPS software. Theantibacterial activityto staphylo-coccus aureus was detected by the microtubule plate method. The results showed that the extraction process of total flavonoids was ethanol concentration of 70%, extract time of one hour, extract temperature of 50 ℃ and solid-liquid ratio of 1：60. Under these conditions, the content of total flavonoids from Polygonum hydropiperwas 5.35%±0.23%. The minimum inhibitory concentration of total flavonoids against Staphylococcus aureus was 0.782 mg/mL. So this extraction process was simple and could be used for extraction of total flavonoids from Chinese medicine polygonum hydropiper. And the extraction had antibacterial activity against staphylococcus aureus.

关 键 词：辣蓼 总黄酮 均匀设计 提取工艺 抑菌活性 

分 类 号：TQ461[化学工程—制药化工]