过表达Rab27A基因对胃癌细胞BGC823生物学行为的影响  

Impact of Rab27A Overexpression on Biological Behavior of Gastric Cancer Cell Line BGC823

作　　者：王倩[1] 杨静文[1] 王飞飞[1] 王如良[1] 李光辉[1] 肖文华[1] 

机构地区：[1]解放军总医院第一附属医院肿瘤内科,北京100037

出　　处：《生物技术通讯》2017年第2期77-83,共7页Letters in Biotechnology

基　　金：国家自然科学基金(81372220)

摘　　要：目的:研究Rab27A与胃癌细胞恶性生物学行为的关系。方法:以Rab27A低表达的胃癌细胞BGC823为出发细胞株,构建Rab27A过表达稳定细胞株,通过MTT实验、软琼脂集落形成实验、Transwell迁移和侵袭实验,观察过表达Rab27A后BGC823细胞增殖、迁移及侵袭等恶性生物学行为的改变。结果:过表达Rab27A后,BGC823细胞的迁移(P<0.05)和侵袭能力(P<0.05)显著降低,但细胞增殖能力没有明显变化(P>0.05)。结论:Rab27A在胃癌细胞发生发展过程中可能作为抑癌基因发挥作用,这一结果为胃癌的临床治疗提供了新的理论依据和潜在的治疗靶点。Objective： To explore the relationship between Rab27A and the malignant biological behavior of gastric cancer cells. Methods： Rab27A was overexpressed in gastric cancer cell line BGC823. MTT, soft agar colony formation and transwell assay were used to observe the malignant behavior change. Results： The migrative and invasive ability of BGC823 cells were obviously reduced after Rab27A overexpression, while the proliferation of BGC823 cell line was not apparently changed. Conclusion： Rab27A might be an anti-oncogene during the devel- opment of gastric cancer, which provide new evidence and potential therapeutic target for the clinical treatment of gastric cancer.

关 键 词：Rab27A 胃癌 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]