左旋龙脑对UVB辐射后小鼠皮肤光损伤的影响  

Eeffects of L-borneol on UVB-induced Photo-damages in Skin of Balb/c Mice

作　　者：李小婷[1,2,3] 王丹[1,2] 庞玉新[1,2,4] 杨全[3] 范佐旺[1,2,3] 马青松[1,2,3] 许罗凤 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [2]海南省艾纳香工程技术研究中心,海南儋州5717373 [3]广东药学院中药学院,广东广州510006 [4]中国中医科学院中药资源中心,北京100700

出　　处：《中国现代中药》2017年第4期518-524,共7页Modern Chinese Medicine

基　　金：科技部科研院所技术开发研究专项(2013EG134239);贵州省重大科技专项计划项目(6011-2013)

摘　　要：目的:研究左旋龙脑对中波紫外线(UVB)照射后小鼠皮肤光损伤的影响。方法:通过UVB照射Balb/c小鼠建立光损伤模型,创面外用左旋龙脑,每天1次,连续给药11 d;在伤后不同时相点取材,HE染色观察皮肤组织的病理变化,对皮肤红斑、水肿反应进行评分;测定左旋龙脑对创面愈合时间、组织含水量、8-OHdG、IL-6和NF-κB的影响。结果:与正常组相比,光损伤小鼠红斑、水肿指数明显增加;与模型组相比,左旋龙脑组红斑、水肿指数明显减轻,结痂脱落时间减少,皮肤组织中8-OHdG、IL-6和NF-κB的水平显著降低(P<0.05,P<0.01);HE染色显示模型小鼠皮肤表皮增厚明显,伴炎性细胞浸润,经左旋龙脑处理后,上述病理改变减轻。结论:左旋龙脑可能通过清除光产物8-OHdG、抑制NF-κB对IL-6的释放的调节作用,使光损伤组织中IL-6下降而起抗炎作用,促进光损伤伤口愈合。Objective： To study effects of L-borneol on interleukin-6 （IL-6）and nuclear factor kappa-B （NF-κB）in UVB irradiation-induced mice. Methods： Eighty of Balb/c mice were randomly divided into four groups. Photo-damaged mouse model was established by UVB irradiation. L-borneol was adopted for wounds once every day for 11 days. The pathological changes of the photo-damaged skin tissue were observed by HE staining. Samples were collected at different time points after UVB irradiation to assess healing time, tissues water content, erythema and edema. The levels of 8-hydroxy-desoxyguanosine （8-OHdG）, IL-6 and NF-κB in the epidermis were detected by ELISA. Results： Compared with the normal group, the erythema and edema indexes increased significantly in the UVB model group. The erythema and edema indexes, IL-6 and NF-κB levels in mice with acute UVB exposure significantly reduced after treatment with L-bomeol. Further more, HE staining showed that UVB irradiation induced keratinoeyte hyperplasia, sunburn cell formation and inflammatory cell infiltration. After treatment with L-bomeol, these histological abnormalities were alleviated. In addition, the topical application of L-borneol resulted in a significant decrease in UVB-induced increases of epidermis thickness, especially depressing in UVB mediated generation of 8-OHdG（P 〈 0. 05, P 〈 0. 01 ）. Conclusion： It is considered that L-borneol could alleviate the symptoms of photo- damages by UVB irradiation. One principle of the effects could be that L-borneol removed of light products 8-OHdG, than controlled NF-κB regulation on release of IL-6 and lead to fall of IL-6 level in photo-damaged tissue causing role of anti-photo-damage.

关 键 词：左旋龙脑 紫外线 光损伤 IL-6 NF—κB 

分 类 号：R57[医药卫生—消化系统]