SDF-1/CXCR4在膀胱癌组织中的表达及其与负性共刺激分子PD-L1、细胞凋亡和侵袭性的相关性研究  

SDF-1/CXCR4 expression in bladder cancer tissue and the correlation with negative costimulatory molecule PD-L1,cell apoptosis and invasion

作　　者：叶明宝[1] 田俊强[1] 常宏[1] 杜昌国 燕群峰 

机构地区：[1]兰州大学第二医院泌尿外科,甘肃兰州730030 [2]陕西省咸阳市第一人民医院泌尿外科,陕西咸阳712000

出　　处：《海南医学院学报》2017年第6期736-738,742,共4页Journal of Hainan Medical University

基　　金：陕西省卫生厅科研基金项目(2010H21)~~

摘　　要：目的:研究SDF-1/CXCR4在膀胱癌组织中的表达及其与负性共刺激分子PD-L1、细胞凋亡和侵袭的相关性。方法:选择在我院手术切除的118例膀胱癌组织和癌旁组织作为研究样本,抽提RNA后反转录为cDNA,检测SDF-1/CXCR4、PD-L1/PD-1以及细胞凋亡相关分子、细胞侵袭相关分子的表达量。结果:膀胱癌组织中SDF-1、CXCR4的mRNA表达量显著高于癌旁组织;膀胱癌组织中PD-L1、PD-1、Rec1、Survivin、MRPS5、Nanog、BCAPP2Ac、TRPM8、TRPV2、ILK、β-catenin、GUGBP1的mRNA表达量显著高于癌旁组织且与SDF-1、CXCR4的mRNA表达量呈正相关。结论:膀胱癌组织中高表达的SDF-1/CXCR4与负性共刺激分子PD-L1、促增殖分子、促侵袭分子的高表达密切相关,SDF-1/CXCR4能够促进膀胱癌细胞的免疫逃逸、增殖以及侵袭。Objective： To study the SDF-1/CXCR4 expression in bladder cancer tissue and the correlation with negative costimulatory molecule PD-L1, cell apoptosis and invasion. Methods： A total of 118 cases of bladder cancer tissue and para-carcinoma tissue surgically removed in our hospital between May 2014 and May 2016 were selected as the research samples, the RNA was extracted and then reverse-transcribed into cDNA, and the expression levels of SDF-I/CXCR4, PD-L1/PD-1, cell apoptosis-related molecules and cell invasion-related molecules were detected. Results： SDF-1 and CXCR4 mRNA expression in bladder cancer tissue were significantly higher than those in para-carcinoma tissue; PD-L1, PD-1, Recl, Survivin, MRPS5, Nanog, BCAPP2Ac, TRPMS, TRPV2, ILK, t3-catenin and GUGBP1 mRNA expression in bladder cancer tissue were significantly higher than those in para-carcinoma tissue and positively correlated with SDF-1 and CXCR4 mRNA expression. Conclusion： Highly expressed SDF-1/CXCR4 in bladder cancer tissue are closely related to the high expression of negative costimulatory molecule PD-L1, pro-proliferation molecules and pro-invasion molecules, and SDF-1/CXCR4 can promote the immune escape, proliferation and invasion of bladder cancer cells.

关 键 词：膀胱癌 基质细胞衍生因子-1 趋化因子受体-4 免疫逃逸 增殖 

分 类 号：R737.14[医药卫生—肿瘤]