食管癌组织中ERCC1和GSTP1表达量与铂类化疗药物敏感性及凋亡、增殖基因表达量的相关性  

Correlation of ERCC1 and GSTP1 expression in esophageal cancer tissue with platinumbased chemotherapy sensitivity as well as apoptosis and proliferation gene expression

作　　者：丁晓权[1] 毕鑑红 马志刚[1] 蒋占鑫 席俊峰[1] 刘鹏[1] 张志斌[1] 

机构地区：[1]陕西省榆林市第一医院胸心外科,陕西榆林719000 [2]陕西省榆林市第一医院消化内科,陕西榆林719000

出　　处：《海南医学院学报》2017年第6期809-812,共4页Journal of Hainan Medical University

基　　金：陕西省抗癌协会科研基金~~

摘　　要：目的:研究食管癌组织中ERCC1和GSTP1表达量与铂类化疗药物敏感性及凋亡、增殖基因表达量的相关性。方法:选择在我院接受PF方案化疗的晚期食管癌患者,化疗前收集食管癌组织并根据化疗后的效果分为化疗敏感组和化疗耐药组,检测食管癌组织中ERCC1、GSTP1以及凋亡基因、增殖基因的表达量。结果:化疗敏感组食管癌组织中ERCC1、GSTP1的蛋白含量以及蛋白阳性表达率均显著低于化疗耐药组,MBP1、DEC1、PTEN的蛋白含量显著高于化疗耐药组,PLCE1、CyclinD1、PAR2的蛋白含量显著低于化疗耐药组;ERCC1、GSTP1阳性表达食管癌组织中MBP1、DEC1、PTEN的蛋白含量显著低于ERCC1、GSTP1阴性表达食管癌组织,PLCE1、CyclinD1、PAR2的蛋白含量显著高于ERCC1、GSTP1阴性表达食管癌组织。结论:食管癌组织中高表达的ERCC1和GSTP1能够降低癌细胞对铂类化疗药物的敏感性,在铂类药物化疗过程中抑制细胞凋亡、促进细胞增殖。Objective： To study the correlation of ERCC1 and GSTP1 expression in esophageal cancer tissue with plati num-based chemotherapy sensitivity as well as apoptosis and proliferation gene expression. Methods.. Patients with advanced esophageal cancer who accepted PF chemotherapy in our hospital between May 2013 and October 2015 were selected, esophageal cancer tissue was collected before the chemotherapy, the patients were divided into chemotherapy sensitivity group and chemotherapy resistance group according to the effect of chemotherapy, and the expression levels of ERCC1, GSTP1 as well as apop tosis and proliferation genes in esophageal cancer tissue were detected. Results： Protein content and positive protein expression rate of ERCC1 and GSTP1 in esophageal cancer tissue of chemotherapy sensitivity group were significantly lower than those of chemotherapy resistance group, MBP1, DEC1 and PTEN protein content were significantly higher than those of chemotherapy resistance group, and PLCE1, CyclinD1 and PAR2 protein content were significantly lower than those of chemotherapy resistance group; MBP1, DEC1 and PTEN protein content in esophageal cancer tissue with positive ERCC1 and GSTP1 expression were significantly lower than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression while PLCE1, Cy- clinD1 and PAR2 protein content were significantly higher than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression. Oonclusions： The highly expressed ERCC1 and GSTP1 in esophageal cancer tissue can decreased the cancer cell sensitivity to platinum-based chemotherapeutics, inhibit cell apoptosis and promote cell proliferation during platinum-based chemotherapy.

关 键 词：食管癌 切除修复交叉互补基因1 凋亡 增殖 

分 类 号：R735.1[医药卫生—肿瘤]