Apatinib联合5-Fu协同抑制乳腺癌MCF-7细胞的体外研究  

Inhibitive Effects of Apatinib and 5-Fu on Breast Cancer Cell Line ECA109 in Vitro

作　　者：安改丽[1,2] 李旭 冯璐[2] 黄尚科 白俊[1] 赵新汉[2] 

机构地区：[1]陕西省人民医院,710068 [2]西安交通大学医学院第一附属医院,710061 [3]陕西省肿瘤医院,710061

出　　处：《实用癌症杂志》2017年第5期702-705,共4页The Practical Journal of Cancer

摘　　要：目的探讨阿帕替尼(Apatinib)联合5-氟尿嘧啶(5-Fu)对乳腺癌MCF-7细胞的抑制作用。方法采用人乳腺癌细胞株MCF-7,首先采用MTT法及应用流式细胞仪测定不同浓度apatinib作用后对其细胞增殖和周期的影响,其次将MCF-7细胞分为4组,即对照组、Apatinib单药组、5-Fu单药组、Apanitib+5-Fu联合组。对各组细胞给予相应药物处理,48 h后分别应用流式细胞仪检测各组药物对MCF-7细胞株凋亡的作用。结果 Apatinib单药对MCF-7细胞有增殖抑制作用,且存在时间剂量依赖关系,而对其细胞周期影响不大。与对照组相比,Apatinib联合5-Fu作用后有协同诱导凋亡作用,经流式细胞仪检测,Apatinib组诱导的细胞凋亡率为12.05%,5-Fu组为25.76%。与单药组比,Apatinib+5-Fu联合组凋亡率升高更为明显,达34.90%(P<0.05)。结论 Apatinib和5-Fu的联合应用在体外协同抑制乳腺癌MCF-7细胞并诱导凋亡,使抗肿瘤活性显著增强。Objective To investigate the inhibitive effects of Apatinib and 5-Fu on breast cancer cell line MCF-7 ceils. Methods MCF-7 cells were treated with Apatinib at different concentrations and different times, cell growth inhibition was assessed by MTT, the cell cycle changes of the cells in response to apatinib treatment were observed by flow cytometry. MCF-7 cells were divided into 4 different groups：the control group, Apatinib group ,5-Fu group and Apatinib ＋ 5-Fu group. The cell apoptosis was detected by flow cytometry via Annexin V-APC/7-AAD double staining after 48 hours. Results Apatinib significantly inhibi- ted the proliferation of MCF-7 ceils in vitro, and the inhibition effects presented with time-and dose-dependent response, but produced no significant effect on the cell cycle. Apatinib ＋ 5-Fu treatment obviously increased the apoptotic rate of the MCF-7 ceils, the difference has statistical significance compared with the control group （ P 〈 0.05 ）. Conclusion Apatinib and 5-Fu can enhance the anti-tumor activity through inducing cell apoptosis.

关 键 词：阿帕替尼 5-氟尿嘧啶 MCF-7 细胞增殖 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]