塞来昔布对脂多糖诱导的RAW264.7巨噬细胞炎症模型炎症因子的影响  

Influence of Celecoxib on inflammatory cytokines of inflammation model in RAW 264.7 macrophages induced by lipopolysaccharide

作　　者：庞逸敏[1] 甘露[1] 王献哲 苏棋 郭哲[2] 何萍[3] 

机构地区：[1]广西医科大学药学院药理学教研室,广西南宁530021 [2]广西医科大学第三附属医院急诊科,广西南宁530021 [3]广西医科大学实验动物中心,广西南宁530021

出　　处：《中国当代医药》2017年第10期4-7,共4页China Modern Medicine

基　　金：国家自然科学基金项目(81360056);广西壮族自治区南宁市科学研究与技术开发计划项目(20153105)

摘　　要：目的建立脂多糖(LPS)诱导的RAW 264.7巨噬细胞炎症模型,探讨塞来昔布对其分泌炎症因子的影响。方法培养小鼠RAW 264.7巨噬细胞,采用1μg/ml的LPS刺激小鼠RAW 264.7巨噬细胞24 h后,收集细胞培养基,采用Griess法测定一氧化氮(NO)及ELISA法测定肿瘤坏死因子α(TNF-α)、前列腺素E_2(PGE_2)的含量,从而建立LPS诱导的RAW 264.7巨噬细胞炎症模型。采用MTT法检测塞来昔布对LPS刺激的RAW 264.7巨噬细胞的毒性作用后,选用8.00μmol/L塞来昔布预处理细胞1 h,再加入1μg/ml LPS刺激细胞24 h,收集细胞培养基,测定培养基中炎症因子NO、TNF-α及PGE_2的含量。结果 1μg/ml的LPS刺激RAW 264.7巨噬细胞24 h后,细胞形态出现明显变形,细胞培养基中炎症因子NO及TNF-α、PGE_2含量均较正常对照组明显增高(P<0.01)。与DMSO溶剂对照组相比,8.00μmol/L塞来昔布组能明显抑制LPS诱导的RAW 264.7巨噬细胞炎性因子NO、TNF-α及PGE_2的释放(均P<0.01)。结论成功建立LPS诱导的RAW 264.7巨噬细胞炎症模型,塞来昔布可明显抑制其炎症因子NO、TNF-α及PGE_2的分泌。Objective To investigate the influence of Celecoxib on the secretion of inflammatory cytokines in RAW 264.7 maerophages model induced by lipopolysaccharide （LPS）.Methods Mouse RAW 264.7 macrophage was cultured. 24 h after mouse RAW 264.7 macrophage was stimulated by LPS 1 μg/ml,then cell culture medium was collected.NO was tested by using Griess and the content of TNF-α and PGE2 was tested by ELISA.Then the RAW 264.7 macrophage inflammation model was established.The toxic effect of celecoxib on LPS stimulated RAW 264.7 macrophages assayed by MTT.8,00 μmol/L Celecoxib was used to pretreat cell for t h,following of 1 μg/ml LPS added to stimulate cell for 24 h. Cell culture medium Was collected to test the content of inflammatory factors NO,TNF-α and PGE2 in culture medium. Results After 1 μg/ml of LPS stimulated RAW macrophages for 24 h,cell morphology changed obviously,the content of inflammatory factors NO,TNF-α and PGE： in culture medium were obvious higher than those of the control group （P〈 0.01）.Compared with DMSO control group,8.00 μmol/L Celecoxib group could significantly inhibit the release of in flammatory cytokines NO,TNF-α and PGE2 induced by LPS in RAW 264.7 cells （P〈0.01）.Conclusion Establishment of LPS induced macrophage model of RAW 264.7 and celecoxib can obvious inhibit the release of inflammatory cytokines NO,TNF-α and PGE2.

关 键 词：塞来昔布 RAW 264.7巨噬细胞 脂多糖 一氧化氮 肿瘤坏死因子α 前列腺素E2 

分 类 号：R-332[医药卫生]