CXCR7沉默对HeLa细胞侵袭和迁移能力的影响及其在宫颈癌中的表达  

The Effects of Inhibiting CXCR7 Gene Expression on Migration and Invasion of HeLa Cells and Expression of Cervical Cancer Tissues

作　　者：王静雯[1] 王思念[2] 孙锁柱[3] 张新[4] 于楠[2] 李峰生[2] 

机构地区：[1]辽宁中医药大学研究生学院,沈阳110032 [2]中国人民解放军火箭军总医院核与辐射损伤实验室 [3]中国人民解放军火箭军总医院病理科 [4]中国医科大学肿瘤医院辽宁省肿瘤医院

出　　处：《国际妇产科学杂志》2017年第2期163-166,I0001,共5页Journal of International Obstetrics and Gynecology

基　　金：辽宁省自然科学基金(2015020287)

摘　　要：目的:探讨趋化因子受体7(CXCR7)沉默对HeLa细胞侵袭及迁移能力的影响及其在宫颈癌及正常宫颈组织中的表达情况。方法:靶向CXCR7的短发夹RNA(shRNA)质粒(CXCR7-shRNA)或对照质粒(Ctrl-shRNA)转染HeLa细胞后,通过Transwell小室法检测细胞侵袭和迁移能力变化。利用免疫组织化学方法检测CXCR7在宫颈癌组织及正常宫颈组织中的表达情况。结果:CXCR7蛋白在20例宫颈癌组织中有16例呈阳性表达。5例正常宫颈组织中CXCR7蛋白表达均呈阴性。Transwell侵袭实验和迁移实验表明,CXCR7-shRNA转染组迁移穿过膜的数量明显低于Ctrl-shRNA转染组和未转染组。结论:宫颈癌组织中特异性高表达CXCR7,抑制宫颈癌细胞CXCR7的表达可显著抑制其侵袭及迁移,因此,CXCR7有望成为宫颈癌治疗的重要靶点。Objective：To investigate the effect of chemokine receptor 7（CXCR7） silencing on invasion and migration of He La cells and its expression in cervical carcinoma and normal cervical tissues. Methods：Respectively, after the He La cells were transfected with plasmid expressing CXCR7 shRNA（CXCR7-shRNA） or negative control shRNA（Ctrl-shRNA）, detecting the changes in cell migration and invasion of them by Transwell method. Immunohistochemistry was used to detect the expression of CXCR7 in cervical cancer tissues and non cancerous cervical tissues. Results：CXCR7 protein was positive in 16 cases of cervical cancer tissrues. Transwell invasion and migration assay showed that the number of CXCR7-shRNA transfected group was significantly lower than that of Ctrl-sh RNA transfection group and non transfection group. Conclusions：The expression of CXCR7 was significantly higher in cervical cancer tissues, and the expression of CXCR7 could inhibit the invasion and migration of cervical cancer cells. Therefore, CXCR7 may be an important target for the prevention and treatment of cervical cancer.

关 键 词：受体 趋化因子 HELA细胞 肿瘤侵润 细胞运动 

分 类 号：R737.33[医药卫生—肿瘤]