热休克蛋白70-甲胎蛋白抗原表位肽疫苗抗小鼠H22肝癌细胞免疫机制的研究  

Study of anti-tumor immunity elicited by peptides complex-heat shock protein 70 and alpha-fetoprotein epitope peptide

作　　者：胥冰[1] 王巧侠[2] 王小平[1] 蔺焕萍[1] 马晓军[1] 张鹏飞[1] 赵倩[1] 张克佩[1] 

机构地区：[1]陕西中医药大学免疫及检验学教研室,咸阳712046 [2]西安市中心医院感染科,西安710003

出　　处：《生物学杂志》2017年第2期46-49,共4页Journal of Biology

基　　金：国家自然科学基金(No.81172135);陕西省自然科学基础研究项目(No.2016JM8023;2016JM8150);陕西省教育厅自然科学基础研究项目(No.07JK233;14JS025)

摘　　要：分别以AFP多肽、HSP70和HSP70-AFP多肽复合物免疫小鼠,检测各组CD8^+T细胞培养上清IFN-γ、TNF-α、穿孔素、颗粒酶B水平及CD8^+T细胞对H22细胞的杀伤作用,探讨热休克蛋白70(HSP70)-甲胎蛋白(AFP)抗原表位肽复合物对小鼠H22肝癌细胞的免疫效应。To study anti-tumor immunity mechanism induced by a reconstructed peptide complex-heat shock protein 70（ HSP70） and alpha-fetoprotein（ AFP）,mice were immunized with recombinant peptide vaccine HSP70-AFP-P,whereas AFP peptide or HSP70 vaccination as controls. IFN-γ,TNF-α and granzyme-B,perforin-produced by separated CD8~＋T cells from vaccinated mice were detected with ELISA,respectively. In vivo tumor loaded test was exerted to evaluate the immune effect of the peptide vaccine. The study suggested that sequential immunization with HSP70-AFP peptide vaccine could bring about effective AFP-specific CD8~＋T cell responses and elicit specific antitumor immunity on AFP-producing tumors.

关 键 词：甲胎蛋白(AFP) 热休克蛋白70(HSP70) 多肽疫苗 抗瘤免疫 

分 类 号：R730.51[医药卫生—肿瘤]