两个红树莓品种的组培快繁技术研究  

Study on Tissue Culture and Rapid Propagation of 2 Red Raspberry Cultivars

作　　者：夏瑞英 岳雷 陆璐[2,3] 柳永霞 王赟 成艳玲 余倩倩[4] 杨伟 XIA Ruiying YUE Lei LU Lu LIU Yongxia WANG yun CHENG Yanling YU Qianqian YANG Wei(Jiuquan Jinshuoyuan Modem Agricultural Development Limited by Share Ltd., Gansu Jiuquan 735000, China Institute of New Rural Development Research, Northwest Normal University, Lanzhou Gansu 730070, China Characteristics of Plant Effective Components of Product Engineering Technology Research Center, Lanzhou Gansu Lanzhou 730070, China College of Horticulture, South China Agricultural University, Guangzhou Guangdong 510642, China)

机构地区：[1]酒泉金硕元现代农业发展股份有限公司,甘肃酒泉735000 [2]西北师范大学新农村发展研究院,甘肃兰州730070 [3]甘肃特色植物有效成分制品工程技术研究中心,甘肃兰州730070 [4]华南农业大学园艺学院,广东广州510642

出　　处：《甘肃农业科技》2017年第4期16-19,共4页Gansu Agricultural Science and Technology

摘　　要：以红树莓品种海尔特兹和秋福的单芽茎段外植体为试验材料,研究了树莓组培快繁各阶段的最佳培养基及出瓶移栽基质。结果表明,初代培养基为MS+6-BA 0.1 mg/L时两个红树莓品种的诱导腋芽萌发效果最好,MS+6-BA 0.5 mg/L+NAA 0.1 mg/L为最佳继代增殖培养基。海尔特兹的最佳生根培养基为1/2MS+IBA 0.1 mg/L+NAA 0.05 mg/L,秋福的最佳生根培养基为1/2MS+IBA 0.2 mg/L+NAA 0.1mg/L。最佳出瓶移栽基质为蛭石、河沙、育苗基质、园土按体积比0.15∶0.15∶0.2∶0.5配制。For the purpose of rapid propagation of 2 cultivars of red raspberry Heritage and Autumn Bliss, media of each propagating stage in vitro and transplanting in soil are optimized and compared. Single-bud-stem cuttings of the two cultivars are used as initial explants in this research. The result shows that the highest initial sprouting rates are achieved with the both varieties on a MS medium supplemented with 6-BA at 0.1 mg/L; for shoot multiplication, a medium composition as MS＋6-BA 0.5 mg/L＋ NAA 0.1 mg/L is the optimal. The best rooting medium for Heritage is 1/2MS＋IBA 0.1 mg/L＋NAA 0.05 mg/L, while the highest rooting ratio for Autumn Bliss was observed on a medium 1/2MS＋IBA 0.2 mg/L＋NAA 0.1 mg/L. Shoots developed good roots invitro are then transplanted into soil and a mixture of 15% vermiculite ＋15% sand＋20% nursery matrix＋50% garden soil is the best transplanting matrix.

关 键 词：红树莓 海尔特兹 秋福 组培快繁 

分 类 号：S723.1[农业科学—林木遗传育种]