Hesperidin Reduces Cisplatin-lnduced DNA Damage in Bone Marrow Cells of Mice  

作　　者：Tatiane da Silva Passos Esdras Andrade Santana Maina Mantovanelli da Mota Jean Carlos Vencioneck Dutra Juliana Macedo Delarmelina Maria do Carmo Pimentel Batitucci 

机构地区：[1]Departamento de Ciencias Biologieas, Universidade Federal do Espirito Santo, Vitoria 29075-91, Brazil

出　　处：《Journal of Pharmacy and Pharmacology》2017年第5期282-288,共7页药剂与药理学（英文版）

摘　　要：Hesperidin is a bioflavonoid abundantly found in citrus fruits and displays chemoprotective effects against DNA （deoxyribonucleic acid） damage, however there are few reports about hesperidin effects against cisplatin-DNA damage induction. The aim of this work was to evaluate hesperidin antimutagenicity against cisptatin-DNA damage. （1） The antimutagenicity of hesperidin was assayed by bone marrow of mice in vivo using the micronucleus test. Hesperidin pre-treatment protocol reduced the frequency of MNPCE （micronucleated polychromatic erythrocytes） and the dose of 100 mg·kg-1 was highest efficiency, with 65.24% of damage reduction. In the simultaneous treatment protocol, the dose of 200 mg·kg-1 exhibited a more effective reduction of MNPCE, with 94.01% of damage reduction. （2） Hesperidin was also effective in reducing the MNPCE frequency in the post-treatment protocol for all doses, with 77.48%, 82.13% and 90.08% of damage reduction at the doses of 100, 200 and 400 mg·kg-1, respectively. From the study, it can be concluded that hesperidin was able to promote the reduction of micronuclei frequency and DNA damage induced by cisplatin. Hesperidin is a powerful antioxidant compound and its chemoprotective effects on DNA may occur due to its association with the antioxidant cell system which is responsible for eliminate free radicals generated by chemical harmful to DNA.

关 键 词：HESPERIDIN micronucleus test DNA damage CHEMOPROTECTION antimutagenicity. 

分 类 号：TS202.3[轻工技术与工程—食品科学] Q523[轻工技术与工程—食品科学与工程]