胆管细胞癌中CtBP1、Zeb1、Zeb2与E-cadherin蛋白的表达及临床意义  

Expression of CtBP1,Zeb1,Zeb2 and E-cadherin in cholangiocarcinoma and their clinical significance

作　　者：胡洁[1] 边立会[2] 王晓玉[2] 杨月丽 张小玲[3] 肖胜军[4] 

机构地区：[1]桂林医学院附属医院病理科,桂林541001 [2]桂林医学院研究生院,桂林541004 [3]桂林医学院基础医学院生理学教研室,桂林541004 [4]桂林医学院第二附属医院病理科,桂林541199

出　　处：《临床与实验病理学杂志》2017年第4期365-369,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81660485);广西壮族自治区和计划生育委员会资助课题(Z2015404)

摘　　要：目的探讨转录抑制因子Zeb1、Zeb2、转录辅抑制因子CtBP1及其靶基因E-cadherin在胆管细胞癌中的表达及CtBP1、Ecadherin的临床意义。方法采用免疫组化法检测胆管细胞癌及癌旁组织芯片中CtBP1、Zeb1、Zeb2和E-cadherin的表达。结果CtBP1在胆管细胞癌及癌旁组织中的阳性率分别为44.44%和17.86%,Zeb2分别为34.92%和10.71%,E-cadherin分别为50.79%和100%,组间差异均有统计学意义(P均<0.05)。Zeb1在胆管细胞癌仅1例表达,而在癌旁组织中不表达。CtBP1与胆管细胞癌的分化程度相关(P<0.05),E-cadherin与胆管细胞癌的分化程度及远处转移有关(P均<0.05)。E-cadherin和CtBP1及Zeb2表达呈负相关(r=-0.034、-0.029,P均<0.001),CtBP1和Zeb2表达呈正相关(r=0.228,P=0.005)。结论胆管细胞癌中CtBP1、Zeb2与E-cadherin均表达异常,CtBP1、Zeb2可能共同参与E-cadherin表达调控。联合检测CtBP1和E-cadherin有望成为评估胆管细胞癌恶性生物学行为的参考指标。Purpose To investigate the expression of transcriptional suppressor CtBP1, Zeb1, Zeb2 and their target gene E-cadherin, and their significance in cholangiocarcinoma. Methods The expression of CtBP1, Zebl, Zeb2 and E-cadherin proteins in cholangioearcinoma and the paired non-neoplastic tissue array were detected by the immunohistohemical staining. Results The positive rates of CtBP1 expression in cholangiocarcinoma and the paired non-neoplastic tissue were 44.44% and 17. 86%, these of Zeb2 were 34.92% and 10. 71%, and these of E-cadherin were 50. 79% and 100%, respectively. The differences between the groups were statistically significant （ all P 〈 0. 05 ）. There was only one case with expression of Zebl in cholangiocarcinoma, but no expression in the paired non-neoplastic tissue. CtBP1 was correlated with the degree of differentiation of cholangiocarcinoma （ P 〈 0.05 ）. Ecadherin was related to the differentiation degree , and distant metastasis of cholangiocarcinoma （ all P 〈 0. 05 ）. The E-cadherin expression was negatively correlated with CtBP1 and Zeb2 （r = - 0. 054, - 0. 029, all P 〈 0.05 ）. The Zeb2 expression was positively correlated with CtBP1 （ r = 0. 228, P = 0. 005 ）. Conclusion CtBP1, Zeb2 and E-cadherin express abnormally in cholangiocarcinoma. CtBP1, Zeb2 may be involved in the regulation of E-cadherin expression. Joint detection of CtBP1 and Ecadherin is expected to be a reference index to evaluate the malignant biological behavior of cholangiocarcinoma.

关 键 词：肝肿瘤 胆管细胞癌 Zeb1 Zeb2 羧基末端结合蛋白1 E-CADHERIN 

分 类 号：R735.8[医药卫生—肿瘤]