外周细胞淋巴瘤中TET2和DNMT3A的表达及意义  

Expression of TET2 and DNMT3A in peripheral T cell lymphoma and their significance

作　　者：孙孟琦 王丽丽[2] 冉雯雯[2] 李宏[2] 邢晓明[2] 

机构地区：[1]青岛大学基础医学院病理学教研室,青岛266071 [2]青岛大学附属医院病理科,青岛266003

出　　处：《临床与实验病理学杂志》2017年第4期388-392,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨TET2和DNMT3A在外周T细胞淋巴瘤(peripheral T-cell lymphoma,PTCL)中的表达及其与PTCL免疫表型的关系。方法应用CD3、CD4、CD10、BCL-6、CXCL-13、CD30、ALK免疫标记将PTCL分为血管免疫母细胞性T细胞淋巴瘤(angioimmu-noblastic Tcelll ymphoma,AITL)、外周T细胞淋巴瘤,非特指(peripheral T-cell lymphoma,not otherwise specified,PTCL-NOS)、ALK阳性的间变性大细胞淋巴瘤(ALK+ anaplastic large cell lymphoma,ALK^+ ALCL)和ALK阴性的间变性大细胞淋巴瘤(ALK-anaplastic large cell lymphoma,ALK^- ALCL)4种类型。应用免疫组化法检测PTCL组织中TET2和DNMT3A的表达。结果 89例PTCL中,AITL 36例,PTCL-NOS 26例,ALK^- ALCL 18例,ALK^+ ALCL 9例。在AITL组织中,TET2胞质表达和DNMT3A胞质及胞核表达的高表达率均高于PTCL-NOS和ALCL,差异有统计学意义(P均<0.05)。TET2胞质表达和DNMT3A胞质及胞核表达在AITL中均呈正相关(P<0.05)。结论 TET2和DNMT3A可以作为诊断AITL的分子标志物,为AITL的明确诊断提供新策略。Purpose To investigate the expression of TET2 and DNMT3A in patients with peripheral T cell lymphoma （PTCL） and the relationship to immunophenotypes of PTCL. Methods Using a panel of immunohistochemical markers （CD3, CD4, CD10, BCL-6, CXCL-13, CD30, ALK）, all eases of PTCLs were further divided into four groups, including angioimmunoblastic T cell lymphoma （AITL）, peripheral T cell lymphoma, not otherwise specified （PTCL-NOS）, anaplastic lymphoma kinase negative anaplastic large cell lymphoma （ALK-ALCL） and anaplastie lymphoma kinase positive anaplastic large cell lymphoma （ ALK ＋ ALCL）. The expression of TET2 and DNMT3A in 89 cases of PTCL was detected by immunohistochemical analysis. Results 89 cases were divide into four subtypes, AITL （36/89）, PTCL-NOS （26/89）, ALK- ALCL （18/89） , and ALK ＋ ALCL （9/89）. Imrnunohistochemistry staining revealed higher cytoplasmic expression of TET2 and DNMT3A in AITL than that of in PTCL-NOS and ALCL （ P 〈 0. 05）. And the nuclear expression of DNMT3A in patients with AITL was higher than that of PTCL-NOS and ALCL （P 〈 0. 05 ）. The cytoplasmic expression of TET2 was positively related with both cytoplasmic and nuclear expression of DNMT3A in patients with AITL （P 〈 0. 05 ）. Conclusion TET2 combined with DNMT3A could he used as markers in AITL diagnosis, which could provide new strategy for AITL diagnosis.

关 键 词：淋巴瘤 外周T细胞淋巴瘤 TET2 DNMT3A 免疫组织化学 

分 类 号：R733.4[医药卫生—肿瘤]