产油脂细菌Bacillus sp. B1的分离与特性研究  

Isolation and Characterization of Lipid-Producing Bacterium Bacillus sp. B1 from Soil

作　　者：任世英[1,2] 邵奎[1] 卢樯 刘飞[3] 

机构地区：[1]淮阴工学院生命科学与食品工程学院,江苏淮安223003 [2]江苏省生物质转化与过程集成工程实验室,江苏淮安223003 [3]淮阴工学院化学工程学院,江苏淮安223003

出　　处：《食品与发酵科技》2017年第2期4-8,共5页Food and Fermentation Science & Technology

基　　金：江苏省淮安市科技计划项目(HAN2015026);江苏省生物质转化与过程集成工程实验室开放课题(JPELBCPL2013004和JPELBCPL2014006)

摘　　要：为了获得产油脂微生物,使用筛选培养基,通过影印平板法和苏丹黑染色法,从河边土壤中分离到一株产油脂细菌,编号为B1。菌落较光滑、乳白色、稍透明、边缘圆润、较粘稠。菌体呈长杆状,G^-,胞内有蓝黑色的油脂颗粒。部分生理生化结果:接触酶、反硝化和荧光色素试验呈阴性,异染粒染色、淀粉水解和卵磷脂试验为阳性。经16S rDNA鉴定属于芽孢杆菌属,暂命名为Bacillus sp. B1。菌株最适培养温度为35℃,起始pH为10。通过盐酸水解法测其产油率为5.29%。In order to obtain microbes producing microbial oil from environment, a strain, marked as B1 was isolated by methods of replica plating and Sudan Black staining on the selected media. Its colony is smooth, milk white, slightly transparent, round edge, sticky. Its cell is long-rod, G-, intracellular lipid granule. The results of physiological and biochemical research： catalase, denitrification and fluorescent pigment tests are negative; metachromatic granule staining, amylolysis, lecithinase tests are positive. According to its sequencing result of 16S rDNA, strain B1 is classification and belongs to genus Bacillus, namely Bacillus sp. B1. The optimum inoculation temperature is 35~C and the most suitable initial pH value is 10. The intracellular lipid of strain B1 is extracted by the way of hydrochloric acid hydrolysis and the calculated ratio of oil-production is 5.29%.

关 键 词：微生物油脂 产油脂细菌 芽孢杆菌属 产油率 

分 类 号：TQ921.1[轻工技术与工程—发酵工程]