红球菌SY095产生物表面活性剂发酵工艺优化及产物稳定性研究  

Study on Optimization of Fermentation Process and Stability of SY095 Produced by Rhodococcus sp

作　　者：张晓青 司晓光 郝建安 姜天翔 任华峰 王静 

机构地区：[1]国家海洋及天津海水淡化与综合利用研究所,天津300192

出　　处：《食品与发酵科技》2017年第2期9-12,43,共5页Food and Fermentation Science & Technology

基　　金：海洋公益性行业科研专项项目(201305022);中央级公益性科研院所基本科研业务费专项资金资助项目(K-JBYWF-2015-G16,K-JBYWF-2015-T11,K-JBYWF-2016-T9)

摘　　要：采用中心组合设计实验和响应面分析法,开展红球菌SY095产生物表面活性剂70L罐的发酵工艺优化,并对提取的生物表面活性剂性质进行研究。通过研究发酵主要影响因子pH和溶氧交互作用,获得生物表面活性剂的最佳发酵方案为:pH 7.89,溶氧32.56%。在该条件下,发酵30h生物表面活性剂粗品产量达17.55g/L,发酵液表面张力从初始的59.235m N/m下降到26.455m N/m。红球菌SY095产生物表面活性剂对环境具有较强适应性,在高温(50-100℃)、高盐(NaCl浓度12%)和较宽pH(2-10)条件下都能保持较高的表面活性,稳定性良好。The large-scale fermentation process in 70 liter fermenter culture of biosurfactant producing by Rhodococcus SY095 was optimized by central composite design and response surface methodology, and the properties of the extracted biosurfactant were studied. By studying the pH and dissolved oxygen interactions which were the main influence factors, the optimum larger-scale fermentation conditions for the biosurfactant were determined as follows： pH 7.89, dissolved oxygen 32.56%. Under the optimum condition, the yield of biosurfaetant could reach 17.55g/L for 30 hours and the surface tension of fermentation supernatant could reduce from culture broth 59.235mN/m to 26.455mN/ m. The biosurfactant producing by Rhodococcus SY095 has strong adaptability to environments. It also demonstrated high surface activity under temperature（50-100℃）, sah（12% NaC1 concentration） and wide adaptable pH（range of 2-10）.

关 键 词：生物表面活性剂 发酵优化 响应面分析 稳定性 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]