云南汉族人群CD40基因多态性与肺癌发生发展的相关性  

The Correlation of CD40 Gene Polymorphisms with the Initiation and Development of Lung Cancer in Yunnan Han Population

作　　者：王小娜[1] 张禹[1] 王培智[1] 周紫云 高雪[1] 杨勤兴 刘舒媛[1] 李传印[1] 史荔[1] 姚宇峰[1] 

机构地区：[1]中国医学科学院医学生物学研究所,云南昆明650118

出　　处：《贵州医科大学学报》2017年第4期383-388,共6页Journal of Guizhou Medical University

基　　金：云南省医学学科带头人培养计划(D-201669);云南省科技厅-昆明医科大学应用基础研究联合专项(2013FB064);云南省肿瘤内设机构(2014NS139)

摘　　要：目的:探讨CD40基因多态性与云南汉族人群肺癌发生发展的相关性。方法:选取云南地区汉族人群肺癌Ⅰ+Ⅱ期患者151例、Ⅲ+Ⅳ期患者316例,同时选择云南地区汉族健康体检人群384例(对照组),采用Taq Man探针基因分型方法对两组被检者CD40基因中rs1883832(C>T)和rs4810485(G>T)2个多态性位点进行基因分型,观察等位基因、基因型及所构建的单倍型在肺癌Ⅰ+Ⅱ期患者、肺癌Ⅲ+Ⅳ期患者和对照组的分布频率。结果:rs4810485位点等位基因G和T在Ⅲ+Ⅳ期肺癌和对照组被检者的分布频率差异具有统计学意义(P=0.013);遗传模式的分析结果经年龄校正后,rs1883832位点在Dominant模式下CC基因型和CT^TT基因型在Ⅰ+Ⅱ期及Ⅲ+Ⅳ期肺癌患者分别与对照组的分布频率比较,差异具有统计学意义(P=0.011和0.008);rs4810485位点在Dominant模式下基因型GG和基因型GT^TTde分布频率比较亦具有统计学意义(P=0.008和0.001)。结论:CD40基因中多态性位点rs4810485位点等位基因G和基因型GG可能是肺癌发生的风险性因素,而rs1883832位点基因型CC可能是肺癌发生的风险因素。Objective： To investigate the correlation of the single nucleotide polymorphisms （SNPs） （rs1883832 C 〉 T and rs4810485 G 〉 T） in CD40 gene with the occurrence and development of lung cancer in a Yunnan Han population. Methods： A total of 155 lung cancer patients in pathological stages Ⅰ ＋ Ⅱ, 316 lung cancer patients in pathological stages Ⅲ ＋ Ⅳ and 384 healthy individuals were recruited in this study. The genotypes of 2 polymorphism loci （rs1883832 C 〉 T and rs4810485 G 〉 T） of CD40 gene were analyzed by Taqman assay. The distribution frequencies of allele, genotype, and haplotypes in lung cancer patients of pathological stages of Ⅰ ＋ Ⅱ and Ⅲ ＋ Ⅳ, and normal control were observed. Results： The allelic frequencies of G and T at locus rs4810485 in group Ⅲ＋ Ⅳ were significantly different from those in control group （P =0. 013 ） , suggesting G at the locus of the allele was the risk factor （OR = 1.33,95% CI： 1.06 - 1.66）. The analysis results of inheritance mode showed that under Dominant mode （best fit mode）, at locus rs1883832, there were significant differences of frequencies of genotypes CC and CT - Tr between ＋ Ⅱ and control, and between Ⅲ ＋ Ⅳ and control group（P =0.011and 0. 008）. In regard of locus rs4810485, under Dominant mode, the distribution frequncies of genotypes of GG and GT - TFde were significantly different between Ⅰ ＋ Ⅱ and control, and between Ⅲ ＋ Ⅳ and control group （ P = 0. 008和 0.001 ）. Conclusions： At polymorphism locus rs4810485 of gene CD40, allele G （ OR = 1.33,95% CI： 1.06 ～1.66） and genotype GG（ OR = 1.79,95% CI： 1.17 ～ 2.74） might be the risk factors of occurrence of lung cancer. While at locus rs1883832, genotype CC be the risk factor of lung cancer initiation（ OR = 1.73,95% CI：1. 13 ～ 2.63）.

关 键 词：基因型 肺肿瘤 相关性 云南 CD40 

分 类 号：R734.2[医药卫生—肿瘤]