携抗MUC1单克隆抗体的超声造影剂微泡制备及体外寻靶实验  

Preparation of Ultrasound Contrast Agent Microbubbles Combining Anti-MUC1 Monoclone Antibody and Evaluation of Targeting Capability in Vitro

作　　者：李小宇[1] 张鹏[1] 罗文[2] 谷芬[2] 周晓东[2] 韩璐[2] 张云飞[3] LI Xiao-yu ZHANG Peng LUO Wen GU Fen ZHOU Xiao-dong HAN Lu ZHANG Yun-fei(Student Bragade, Fourth Military Medical University Department of Ultrasound, Xijing Hospital, Fourth Military Medical University, Xi＇an, Shaaturi, 710032, China Department of Orthopaedics, Tangdu Hospital, Fourth Military Medical University Xi＇an, Shaanxi, 710032, China)

机构地区：[1]第四军医大学学员旅,陕西西安710032 [2]第四军医大学西京医院超声科,陕西西安710032 [3]第四军医大学唐都医院骨科,陕西西安710032

出　　处：《现代生物医学进展》2017年第10期1815-1817,1917,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81000918);陕西省自然科学基金项目(2014JQ4166)

摘　　要：目的:制备携抗MUCI单克隆抗体的超声造影剂微泡,并评价其体外寻靶能力。方法:采用异型双功能交联剂将小鼠抗MUCI单克隆抗体与超声造影剂微泡相结合,采用激光粒度仪,扫描电镜及激光共聚焦显微镜评价其粒径,形态及结合率。体外培养小鼠EMT6细胞,将其与靶向微泡相混合,激光共聚焦显微镜评价粘附能力。结果:扫描电镜下显示携抗MUCI单克隆抗体造影剂微泡呈规则球体形态,平均粒径2.88±1.34μm,通过异型双功能交联剂,抗MUC1单克隆抗体可结合至超声造影剂微泡表面,其结合率为77.3±10.4%,靶向造影剂粘附体外培养细胞比例为79.2±13.2%。结论:成功制备携抗MUCI的靶向超声造影剂微泡,并且能很好的识别粘附体外培养乳腺癌细胞。Objective： To prepare ultrasound contrast agent microbubbles combining anti-MUC1 monoclone antibody and evaluate its targeting capability in vitro. Methods： Ultrasound contrast agent microbubbles and anti-MUC1 monoclone antibodies were combined via N-succinimide 3- （2-pyridyl dithio） propionate （SPDP）. Laser particle size analyzer, scanning electron microscope （SEM） and laser scanning confocal microscope （LSCM） were used to assess the diameter, morphology and ratio of combination between microbubbles and antibodies. Mice EMT6 breast cancer cells were cultured in vitro, which were then mixed with MUCl-targeted microbubbles. Adhension rate were evaluated via LSCM. Results： SEM showed MUCl-antibody carrying microbubbles were regular spherical particles with the diameter of 2.88±1.34μm. Via SPDP, MUCl-antibodies were conjugated onto the surface ofmicrobubbles with the ratio of 77.3± 10.4% by LSCM. Adhension rate was 79.2 ±13.2%, when MUCl-antibody carrying microbubbles were mixed with Mice EMT6 breast cancer cells cultured in vitro. Conclusions： MUCl-antibody carrying microbubbles were prepared successfully, and could adhere to breast cancer cells in vitro.

关 键 词：MUC1 超声造影剂 乳腺癌 

分 类 号：R-33[医药卫生] R445.1