结膜松弛症结膜组织及泪液中黏蛋白的表达变化  

Study on the expression of mucin in conjunctival tissue and tear in eyes with conjunctivochalasis

作　　者：项敏泓[1] 陈星星[1] 周欢明[1] 贾元玲 李青松[1] 张兴儒[1] 盛霞[2] 

机构地区：[1]上海中医药大学附属普陀医院眼科,中国上海市200062 [2]上海中医药大学附属普陀医院病理科,中国上海市200062

出　　处：《国际眼科杂志》2017年第5期857-860,共4页International Eye Science

基　　金：上海市卫生及计划生育委员会资助项目(No.20124108);上海市普陀区自主创新科研基金(No.2013PTKW009)~~

摘　　要：目的:观察结膜松弛症松弛结膜组织和泪液中黏蛋白的表达变化,探讨结膜松弛症的发病机制。方法:收集结膜松弛症组38例38眼和正常对照组36例36眼的结膜组织和泪液标本,分别行免疫组织化学染色检测结膜组织中黏蛋白(MUC2、MUC4、MUC5AC、MUC16)表达情况,ELISA检测泪液中黏蛋白(MUC2、MUC4、MUC5AC、MUC16)A值,结果行统计学分析,比较两组之间的差异。结果:结膜松弛症组的结膜上皮细胞中MUC2、MUC4与正常对照组表达无统计学差异(P=0.315、0.156);结膜松弛症组的结膜上皮细胞中MUC5AC、MUC16阳性表达细胞较正常对照组明显减少,差异有统计学意义(P=0.016、<0.01)。结膜松弛症组泪液中MUC2的A值与正常对照组无统计学差异(P=0.651),结膜松弛症组中MUC4、MUC5AC的A值较正常对照组明显降低,有显著的统计学差异(均P<0.01),结膜松弛症组中MUC16的A值较正常对照组下降,差异有统计学意义(P=0.022)。结论:结膜松弛症患者结膜组织和泪液中MUC5AC和MUC16均下降,对其进一步研究可能揭示结膜松弛症的发病机制。AIM： To investigate the expression of mucin in the conjunctival tissue and tear in eyes with conjunctivochalasis（CCh）. METHODS： Thirty-eight cases（38 eyes）of CCh were recruited as the CCh group and 36 cases（36 eyes）without CCh were included as the normal control group and the conjunctival tissue and tear were collected, respectively. Mucins（MUC2, MUC4, MUC5AC, MUC16）expression in conjunctival tissue were detected by immunohistochemical staining; Mucins（MUC2, MUC4, MUC5AC, MUC16）OD value in tear were determined by ELISA, the differences of which were compared between the CCh and normal control group. RESULTS： No difference in the expression of MUC2 and MUC4 in conjunctival epithelia was observed between the two groups（P=0.315, P=0.156）. There was significant difference in the expression of MUC5AC and MUC16 in conjunctival epithelia between the two groups（P=0.016, P〈0.01, respectively）. No significant difference in the OD value of MUC2 in tear was found between the two groups（P=0.651）. The OD value of MUC4 and MUC5AC in tear were significantly lower in CCh group that in the normal control group（P〈0.01, P〈0.01, respectively）, so was the OD value of MUC16（P=0.022）. CONCLUSION： MUC5AC and MUC16 both decreased in the conjunctiva and tear of eyes with CCh. The further study may reveal the pathogenesis of conjunctivochalasis.

关 键 词：结膜松弛症 黏蛋白 结膜组织 泪液 

分 类 号：R777.3[医药卫生—眼科]