奥氮平对MK-801处理大鼠自发活动和额叶微小RNA-219表达的影响  

Effects of Olanzapine on Locomotor Activity and the Expression of Micro RNA-219 in Frontal Cortex of MK-801-treated Rats

作　　者：高振勇[1] 杨平[1] GAO Zhen-yong YAN GPing(Department of Psychiatry, Suzhou Psychiatric Hospital, Suzhou 215008, China)

机构地区：[1]苏州市广济医院精神科,江苏苏州215008

出　　处：《汕头大学医学院学报》2017年第1期20-21,25,共3页Journal of Shantou University Medical College

基　　金：苏州市"科教兴卫"青年科技资助项目(KJXW2013034)

摘　　要：目的:探讨非典型抗精神病药奥氮平对N-甲基-D-天门冬氨酸(NMDA)受体拮抗剂MK-801处理大鼠自发活动和额叶微小RNA(miRNA)-219表达的影响。方法:将18只成年雄性SD大鼠随机分为对照组(生理盐水(NS)灌胃+NS腹腔注射)、MK-801组(NS灌胃+MK-8010 1 mg/kg腹腔注射)及奥氮平组(奥氮平2mg/kg灌胃+MK-8010 1 mg/kg腹腔注射):每日给药1次,1周后做开场实验检测大鼠自发活动,用实时荧光定量PCR技术检测各组大鼠额叶miRNA-219的表达。结果:与对照组比,MK-801组大鼠自发活动增加,额叶miRNA-219表达下降,差异均有统计学意义(P<0.01);而与MK-801组比,奥氮平组大鼠自发活动减少,额叶miRNA-219表达增加,差异有统计学意义(P<0.05或P<0.01)。结论:奥氮平能抑制大鼠自发活动的增加及额叶miRNA-219的下调。Objective：To investigate the effects of olanzapine on locomotor activity and the expression of microRNA （miR） -219 in frontal cortex of rats treated by a N-methyl-D-aspartate（NMDA） receptor antagonist, MK-801. Methods： 18 adult male SD rats were randomly divided into three groups（n=6）：control group（salinei.g.＋salinei.p.）, model group（saline i.g.＋MK-801 0. lmg/kg i.p.）,olanzapine＋MK-801 group（olanzapine 2mg/kg i.g.＋MK-8010. lmg/kgi.p.）.After rats in each group got the corresponding treatment once per day for a week,open field test was used to detect locomotor activities and Quantitative Real-time PCR to detect the expression of miRNA-219 in prefrontal cortex of rats.Results： Compared with control group,the locomotor activity in MK-801 group increased and the expression of miRNA-219 decreased significantly（P〈0.01）; and control group as well the locomotor activity in olanzapine＋MK- 801 group decreased and the expression of miRNA-219 of increased significantly（P〈0.05 or P〈0.01）. Conclusions： Olanzapine can inhibite the increase of locomotor activity and the decrease of miRNA-219 in frontal cortex of rats.

关 键 词：奥氮平 MK-801 N-甲基-D-天门冬氨酸 微小RNA-219 额叶 

分 类 号：R749.3[医药卫生—神经病学与精神病学]