无菌大鼠培育的技术创新及其繁育探讨  

Technical Innovation and Breeding Research in Germ-free Rats

作　　者：朱祥宇[1] 刘君芳[1] 周淑佩[1] 张阔[1] 王学文[1] 郑振辉[1] ZHU Xiang-yu LIU Jun-fang ZHOU Shu-pei ZHANG Kou WANG Xue-wen ZHENG Zhen-hui(The Department of Laboratory Animal Science, Health Science Center, Peking University, Beijing 100191, China)

机构地区：[1]北京大学实验动物科学部,北京100191

出　　处：《实验动物科学》2017年第1期57-61,共5页Laboratory Animal Science

基　　金：国家自然科学基金(No.81573118)

摘　　要：对清洁级SD大鼠进行剖腹取胎净化,经人工饲育建立无菌SD大鼠群,以满足科研单位对无菌大鼠的需求。在手术隔离器中剥离子宫,取35只仔鼠传入饲育隔离器,静置2 h后存活20只;用自行研制的灌胃注射器,和市售配方改良奶粉,对剖腹取胎仔鼠进行人工哺乳。每24 h人工哺乳5~6次。哺乳20只,离乳13只。粪便、垫料、棉拭子经无菌检查,均无细菌生长。通过优化人工乳配方,创新无菌鼠培育技术,成功建立无菌大鼠模型,并传代保种,解决了科学研究在国内买不到无菌大鼠的难题。The clear grade Sprague Dawley rat was purified by cesarean birth and a gnotobiotic population was established by breeding,it will meet requirement of scientific research units,the 35 offspring were obtained by cesarean birth in isolation environment. Two hours later,survival 20 offspring were feed the modified milk by intragastrical gavages and self-designed equipment. The feeding rate was 5 ~ 6 times a day. the 13 offspring were successful wean. These gnotobiotic animals were verified by detecting excrement padding and cotton swab,the gnotobiotic rat model was successful established by germ-free technology and modified milk,which could be conserved by breeding. It solves the problem of shortage of gnotobiotic rat.

关 键 词：大鼠 剖宫取胎 人工哺乳 隔离环境 无菌检查 

分 类 号：Q95-33[生物学—动物学]