小鼠缺血性脑卒中后免疫抑制及其机制  

Changes and Mechanisms of Immunodepression after Ischemic Stroke in Mice

作　　者：谢姝姮 王进昆[1] 王崇谦[1] 陈雪菲[1] 高锋[1] 汤志伟[1] XIE Shu-heng WANG Jin-kun WANG Chong-qian CHENXue-fei GAO Feng TANG Zhi-wei(Dept. of Neurosurgery, The 1st Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650032,)

机构地区：[1]昆明医科大学第一附属医院神经外科,云南昆明650032

出　　处：《昆明医科大学学报》2016年第7期6-9,共4页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81360204);云南省卫生科技计划项目(2014NS178)

摘　　要：目的研究C57BL/6小鼠肺组织及脾脏对缺血性脑卒中的反应情况,探讨缺血性脑卒中后免疫功能的变化及其变化机制.方法实验分析16只C57BL/6小鼠,随机分为实验组(n=8)和对照组(n=8),实验组采用大脑中动脉线栓法(middle cerebral artery occlusion,MCAO)建立缺血性脑卒中模型,对照组不做处理.以HE染色法观察分析肺组织炎症反应情况,测量比较2组小鼠的脾脏重量、脾脏指数、脾细胞数量及其凋亡情况.结果与对照组相比,实验组小鼠肺组织炎症细胞浸润增多,脾脏重量、脾脏指数及脾细胞数量减少,脾细胞凋亡增多.结论缺血性脑卒中模型小鼠肺部发生炎症反应,脾脏缩小,脾细胞凋亡增加,提示脑卒中后可能通过促进免疫细胞的凋亡而导致脑卒中后的外周免疫抑制,导致机体并发感染.Objective To investigate the changes and mechanisms of immune function after ischemic stroke by studying the reaction of C57BL/6mice＇s lung and spleen after ischemic stroke. Methods Sixteen mice C57BL/6 were randomly assigned to experiment group （n----8） and control group （n=8） . The acute ischemic stroke model was established in experiment group by Middle Cerebral Artery Occlusion （MCAO） and no stroke was performed in control group. Then we observed the lung inflammation by HE staining of the lung. And we measured the spleen weight, spleen weight index, the count and the apoptosis index of spleen cells of mice in two groups. Results Compared with the control group, the count of the inflammatory cells in lung of mice in the experiment group was higher, the spleen weight, spleen weight index and spleen cells count of the experiment group were decreased and the apoptosis index of spleen ceils of the experiment group was higher. Conclusion The mice in the experiment group have inflammatory reaction in lung, the spleen atrophied and the apoptosis index of spleen cells is increased, which suggest that stroke-induced apoptosis of immunocytes may cause immunodepression after ischemic stroke and easily lead to the infection.

关 键 词：缺血性脑卒中 脾脏 淋巴细胞 免疫抑制 凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]