^(99m)Tc-MIBI显像评价小鼠Lewis肺癌多药耐药的实验研究  

Experimental Study on ^(99m)Tc-MIBI Imaging Evaluating Multidrug Resistance of Mice Lewis Lung Cancer

作　　者：刘超[1] 陈婷[1] 吕娟[1] 王熙才[2] 邓智勇[1] LIU Chao CHEN Ting LV Juan WANG Xi-cai DENG Zhi-yong(Dept. of Nuclear Medicine Tumor Research Center, Yunnan Provincial Tumor Hospital, Kunming Yunnan 650118, China)

机构地区：[1]云南省肿瘤医院暨昆明医科大学第三附属医院核医学科,云南昆明650118 [2]云南省肿瘤医院暨昆明医科大学第三附属医院肿瘤研究所,云南昆明650118

出　　处：《昆明医科大学学报》2016年第8期9-13,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81160401;30660212);云南省科技厅应用基础研究基金资助项目(2013FB170;2013FB103)

摘　　要：目的采用^(99m)Tc-甲氧基异丁基异腈(MIBI)SPECT显像技术在C57BL/6J小鼠Lewis肺癌的模型上观察应用人参皂苷Rg3(以下简称Rg3)前后的肿瘤细胞摄取显像剂的情况,以探讨应用该方法评价肿瘤多药耐药(MDR)状态的可行性.方法将小鼠Lewis肺癌模型随机分为应用Rg3组和对照组,在应用Rg3前后分别进行^(99m)Tc-MIBISPECT显像及感兴趣区(ROI)半定量分析,观察肿瘤多药耐药状态变化情况,并将其结果与流式细胞仪检测结果进行对比.结果对照组与应用Rg3组显像、应用Rg3前显像与应用Rg3后显像之间15、60、120min的肿瘤摄取比值(T/N)差异有统计学意义(P<0.01).对照组和Rg3组清除指数(WR)均与流式细胞仪检测P-gp蛋白表达阳性细胞的百分率成正相关(P<0.05).结论 ^(99m)Tc-MIBI显像与小鼠Lewis肺癌细胞P-糖蛋白(P-gp)的表达成负相关,可以较好地显示肿瘤的多药耐药状态,同时也可动态监测Rg3对小鼠Lewis肺癌多药耐药逆转的效果.Objectives 99mTc- methoxyisobutylisonitrile（MIBI） SPECT imaging technology was used to observe the condition of tumor cell in- taking imaging agent in the C57BL/6J mice Lewis lung cancer model before and after using Ginsenoside Rg3（short for Rg3）.We aimd so as to discuss the feasibility of using this method to evaluate tumor multidrug resistance（MDR） status. Me thods Mice Lewis lung cancer models were randomly divided into Rg3 group and the control group. After applying Rg3, semi- quantitative analysis was made on 99mTc- MIBI SPECT imaging and region of interest（ROI） to observe the multidrug resistance state of tumor and then the results were compared with the detection results of flow cytometry. Re s ults The tumor intake ratio（T/N） difference between the control group and the Rg3 group in imaging, imaging before applying Rg3 and imaging after applying Rg3 were separately 15, 60 and 120 min.The differences were statistical significant（P 〈 0.01）. The eliminate indexes（WR） of the control group and Rg3 group were positively related to P- gp protein expression positive cells detected by flow cytometry（P 〈 0.05）. Conclus ions 99mTc- MIBI imaging is negatively related to P- glycoprotein（P- gp） expression in mice Lewis lung cancer cells,which can clearly show the multidrug resistance state of tumors and dynamically monitor the effect of Rg3 on multidrug resistance reversion of mice Lewis lung cancer.

关 键 词：99MTC-MIBI 肺肿瘤 多药耐药 P-GP 流式细胞术 

分 类 号：R734.2[医药卫生—肿瘤]