细菌性阴道炎与8种牙周可疑致病微生物感染的关系  

The Relationship between the Bacterial Vaginosis and 8 Putative Periodontal Pathogen Infection

作　　者：李玉华[1] 张明珠[1] 彭艺[1] 徐杰[1] 税艳青[1] LI Yu-hua ZHANG Ming-zhu PENG Yi XU Jie SHUI Yan-qing(Dept. of Periodontalogy, The Affiliated Stomatalogy Hospital of Kunming Medical University, Kunming Yunnan 650100, China)

机构地区：[1]昆明医科大学附属口腔医院牙周科,云南昆明650100

出　　处：《昆明医科大学学报》2016年第9期9-13,共5页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资项目(2014FZ039)

摘　　要：目的探讨8种牙周可疑致病菌感染和细菌性阴道炎的关系.方法选取48例细菌性阴道炎和47例阴道健康患者.采集其阴道分泌物和牙周龈下菌斑,通过DNA提取和二步PCR的方法检测8种牙周可疑致病菌,即牙龈卟啉单胞菌(porphyromonasgingivalis,P.g)、福赛坦氏菌(tannerella forsythia,T.f)、齿垢密螺旋体(treponemasdenticola,T.d)、中间普氏菌(prevotellaintermedia,P.i)、变黑普氏菌(prevotellanigrescens,P.n)、微小消化链球菌(peptostreptococcus micros,P.m)、具核梭杆菌(fusobacteriumnucleatum,F.n)和直肠弯曲杆菌(campylobacter rectas,C.r)在其中的分布情况.同时记录社区牙周指数规定牙位的菌斑指数(plaque index,PLI)、探诊出血(bleeding on probing,BOP)、探诊深度(probing depth,PD)和临床附着丧失(clinical attachment loss,CAL).结果实验组的牙周病患病率、PLI、BOP和CAL明显高于对照组(P<0.05),PD在2组之间无明显差异(P>0.05);实验组和对照组的龈下菌斑和阴道分泌物中均检测出8种牙周致病微生物;T.d在实验组龈下菌斑和阴道分泌物中的检出率均明显高于对照组(P<0.05);其余7种微生物在2组之间检出率均无明显差异(P>0.05).结论细菌性阴道炎患者的牙周病患病率较高;8种牙周致病菌可能是龈下菌斑和阴道分泌物中的常驻菌群.T.d感染可能与细菌性阴道炎的发生有关.Objective To investigate the relationship between the bacterial vaginosis and 8 putative periodontal pathogen infection.Methods A total of 48 patients with bacterial vaginosis were collected and 47 healthy female patients were selectedin the control group. Vaginal secretion and subgingival plaque were obtained from the study group and the control group. The presence of Porphyromonasgingivalis（P.g）, Tannerella forsythia（ T.f）, Treponemas denticola（ T.d）, Prevotell intermedia（ P.i）, Prevotella nigrescen（ P.n）,Peptostreptococcus micros（P.m）,Fusobacterium nucleatum（F.n） and Campylobacter rectas（C.r） was detected by DNA extraction and PCR method. Simultaneously,all the patients underwent a clinical periodontal examination of the teeth in community periodontal index, including plaque index（PLI）,bleeding on probing（BOP）,probing depth（PD） and clinical attachment loss（CAL）.Results The prevalence of periodontal disease, PLI,BOP and CAL in the study group was significantly higher than in the control group（P〈0.05） and the PD had no statistically significant difference between the 2 groups（P〉0.05）.The 2 groups were both detected 8 putative periodontal pathogens in the vaginal secretion and the subgingival plaque samples. The detection ratio of T.dfrom boththe vaginal secretion and subgingival plaque samples was significantly higher in the study group than that in the control group（P 〈0.05）, and other 7 pathogens showed no statistically significant difference（P〈 0.05）.Conclusion The prevalence of periodontal disease was higher among the bacterial vaginosis patients. The 8 putative periodontal pathogens were normal flora in the subgingival plaque and vaginal secretion.Td might be relevant to the pathogenesis of bacterial vaginosis.

关 键 词：牙周可疑致病菌 细菌性阴道炎 阴道分泌物 龈下菌斑 

分 类 号：R780.4[医药卫生—口腔医学]