多囊肾病小型猪肾脏组织中增殖和凋亡的改变  

Changes of Proliferation and Apoptosis in the Kidney of Mini-Pigs with Autosomal Dominant Polycystic Kidney Disease

作　　者：连晓英 白雪源[1] 赵静[1] 张英杰[1] 曹静[1] 王媛媛[1] LIAN Xiao-.ying BAI Xue-yuan ZHAO Jing ZHANG Ying-jie CAO Jing WANG Yuan-yuan(Department of Nephrology, State Key Laboratory o~Kidney Diseases, National Clinical Research Center for Kidney Diseases, Chinese PLA General Hospital, Beijing, 100853, China)

机构地区：[1]解放军总医院肾病科,肾脏疾病国家重点实验室,国家慢性肾病临床医学研究中心,北京100853

出　　处：《现代生物医学进展》2017年第11期2007-2011,2045,共6页Progress in Modern Biomedicine

基　　金：国家重点研发计划项目(2016YFA0101002);国家自然科学基金项目(81570659);海南省社会发展科技专项资金项目(SF201341)

摘　　要：目的:观察ADPKD小型猪肾脏组织中细胞增殖和凋亡的改变。方法:使用PKD1基因敲除的小型猪模型,用Western blot和免疫组化方法检测肾脏组织中细胞增殖指标PCNA;增殖相关的mTOR信号通路分子(phospho-mTOR、phospho-p70S6、phospho-4EBP1)和ERK信号通路分子(phospho-PKA、phospho-MEK、phospho-ERK)的表达水平以及内皮细胞粘附分子CD31以及凋亡相关蛋白Bcl-2、Bax、caspase 3的表达变化。结果:在ADPKD小型猪肾脏组织中,增殖指标PCNA表达显著升高。mTOR信号通路分子phospho-mTOR、phospho-p70S6、phospho-4EBP1水平明显升高,ERK信号通路分子中phospho-PKA、phospho-MEK、phospho-ERK水平明显升高。CD31表达明显升高,Bax/Bcl-2的比值以及caspase 3的表达水平显著升高。结论:本研究显示在ADPKD小型猪肾脏组织中,细胞增殖和凋亡信号通路明显激活。Objective： To observe proliferation and apoptosis of the cell in the kidney of Mini-Pigs with ADPKD. Methods： We observed the protein expression changes of cell proliferation marker PCNA, proliferation-related mTOR signal pathway key molecules （phospho-mTOR, phospho-p70S6, phospho-4EBP 1）, ERK signal pathway key molecules （phospho-PKA, phospho-MEK, phospho-ERK）, endothelial cell adhesion molecule-1 （CD31）, Bax, Bcl-2 and caspase 3 in the kidney tissues of PKD1 deletion mini-pig model by West-ern blot and Immunohistochemical staining. Results： The results showed that the level of PCNA was markedly up-regulated. The molecules in mTOR signal pathway （phospho-mTOR, phospho-p70S6, phospho-4EBP1）, ERK signal pathway （phospho-PKA, phos- pho-MEK, phospho-ERK） and CD3 lwere markedly increased in ADPKD mini-pig kidney tissues. In addition, the Bax/Bcl-2ratio and the caspase3 were increased significantly. Conclusion： The research shown that the signal pathway of proliferation and apoptosis was activated in ADPKD mini-pig kidney tissues.

关 键 词：常染色体显性多囊肾病 增殖 凋亡 小型猪 

分 类 号：R-33[医药卫生] R692