毛细管柱气相色谱内标法同时测定白酒中11种成分  

Simultaneous determination of eleven compounds in Chinese liquor by gas chromatography-internal standard method

作　　者：邱凤梅[1] 陈伟[1] 虞吉寅[1] 程畿 

机构地区：[1]岱山县疾病预防控制中心,浙江岱山316021

出　　处：《中国卫生检验杂志》2017年第7期948-950,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立毛细管柱气相色谱内标法测定白酒中甲醇、杂醇油、酯类等11种成分的分析方法,以便快速、高效、准确的测定白酒中多种成分物质,满足日常检测工作的需要。方法利用气相色谱法,通过程序升温模式,以DBWAX柱(30 m×0.25μm×0.32 mm)为分离柱,火焰离子化检测器(FID)检测,以保留时间定性,乙酸正丁酯内标标准曲线法定量。结果 11种物质在0.02%~1.0%(V/V)线性范围内,相关系数≥0.998 9;样品加标回收率为90.0%~102%,精密度试验的相对标准偏差为0.11%~1.05%,方法检出限为0.001 g/L^0.010 g/L。结论该法操作简单、稳定可靠,实现了白酒中11种成分良好的分离效果和实验精密度,满足检测的要求,可以应用到日常白酒样品的检测分析中。Objective To establish a gas chromatography-internal standard method for the determination of 11 components including methanol,fusel oil and,esters in Chinese liquor.For quick,efficient and accurate determination of many ingredients in liquor to meet the needs of daily inspection work.Methods By means of gas chromatography,the samples were separated with DB-WAX capillary column（30 m × 0.25 μm × 0.32 mm） throughing a temperature programmed mode; the flame ionization detector（FID） was used to determine the retention time; the quantitative determined by standard curve of internal n-butyl acetate.Results The method had a good linearity in the range of 0.02%-1.0%（V/V） with the correlative coefficients ≥0.998 9.The additive recoveries were within 90.0%-102%,the relative standard deviations were within 0.11%-1.05%,and the method detection limits were within 0.001 g/L-0.010 g/L.Conclusion The method is simple,stable and reliable.It can achieve good separation effect and experimental precision of 11 components in liquor,and can meet the requirements of detection.

关 键 词：毛细管柱气相色谱法 内标法 白酒 

分 类 号：O657.71[理学—分析化学]