睡眠剥夺及恢复对大鼠海马GAT-1表达的影响  

EFFECTS OF SLEEP DEPRIVATION AND RECOVERY ON THE EXPRESSION OF GAT-1 IN HIPPOCAMPUS OF RATS

作　　者：张丽伟[1] 丁明[2] 迟蕾[3] 耿聪[1] 

机构地区：[1]潍坊医学院麻醉学系,山东潍坊261053 [2]中国人民解放军第89医院麻醉科 [3]山东省文登整骨医院麻醉科

出　　处：《齐鲁医学杂志》2017年第1期57-59,62,共4页Medical Journal of Qilu

摘　　要：目的探讨睡眠剥夺及恢复对大鼠海马γ-氨基丁酸转运体-1(GAT-1)表达的影响。方法健康雄性SD大鼠24只,随机分为正常对照组(C组)、睡眠剥夺组(D组)和睡眠剥夺+恢复组(D+R组),每组8只。各组大鼠实验前进行Morris水迷宫训练,C组于实验第5天行水迷宫测试;D组睡眠剥夺96h,D+R组睡眠剥夺96h后恢复睡眠12h,然后行水迷宫测试。测试完毕立即处死大鼠,用免疫组织化学法检测海马CA1区GAT-1的表达。结果与实验前比较,D组、D+R组逃避潜伏期延长(t=-5.714、-3.069,P<0.05)。与C组比较,实验后D组、D+R组逃避潜伏期延长(F=21.535,q=9.23、3.76,P<0.05),穿越原平台次数减少(F=19.096,q=8.72、3.88,P<0.05),D组海马CA1区GAT-1阳性细胞数减少(F=21.664,q=4.77,P<0.05);与D组比较,实验后D+R组逃避潜伏期缩短(q=5.48,P<0.05),穿越原平台次数和海马CA1区GAT-1阳性细胞数增加(q=4.85、4.54,P<0.05)。实验前3组逃避潜伏期差异无显著性(P>0.05)。结论睡眠剥夺及恢复对大鼠空间学习记忆能力的影响与海马中GAT-1的表达有关。Objective To investigate the effects of sleep deprivation and recovery on the expression ofγ-aminobutyric acid transporter-1（GAT-1）in hippocampus of rats. Methods Twenty-four healthy male Sprague Dawley rats were equally randomized to three groups as normal control group（group C）,sleep deprivation group（group D）and sleep deprivation ＋ recovery group（group D＋R）.Morris water maze traing was conducted in rats of each group before the experiment.The rats in group C received Morris water maze test on the fifth day of the experiment.The rats in group D were deprivated from sleeping for 96 h and then received Water maze test,those in group D＋R were deprivated from sleeping for 96 h,followed by recovery of sleep for12 h,and then Water maze test carried out.The rats were executed upon completion of the test.Using immunohistochemistry method,the expression of GAT-1in the hippocampus CA1 region was detected. Results Compared with before the experiment,the escape latency in group D and group D＋R was prolonged（t=-5.714,-3.069;P〈0.05）.After the experiment,compared with group C,the escape latency in groups D and D＋R prolonged（F=21.535;q=9.23,3.76;P〈0.05）,the frequency of crossing the original platform decreased（F=19.096;q=8.72,3.88;P〈0.05）,and the number of GAT-1positive cells in the hippocampus CA1 region in group D decreased（F=21.664,q=4.77,P〈0.05）.Compared with group D,the escape latency in group D＋R was shor-tened（q=5.48,P〈0.05）,the frequency of crossing the original platform and the number of GAT-1positive cells in the hippocampal CA1 region increased in group D＋R after sleep recovery（q=4.85,4.54;P〈0.05）.Before the experiment,the difference in the escape latency among the three groups was not significant（P〉0.05）. Conclusion The effect of sleep deprivation and recovery on the spatial learning and memory ability of rats is related to the expression of GAT-1in hippocampus.

关 键 词：睡眠剥夺 海马 Γ-氨基丁酸 氨基酸转运系统 

分 类 号：R740[医药卫生—神经病学与精神病学]