食管胃交界部腺癌中MGMT基因启动子区甲基化状态对预后的影响  

Methylation Status of MGMT Gene Promoter in Patients with Adenocarcinoma of Esophagogastric Junction and Its Relationship with Prognosis

作　　者：李宝重[1] 王文豪[2] 何明[3] 陈新[3] 朱辉[3] 徐新建[3] 李飞[3] 张玉芬[3] 

机构地区：[1]首都医科大学附属北京世纪坛医院胸外科,北京100038 [2]河北省人民医院胸外科,石家庄050051 [3]河北医科大学第四医院胸外科,石家庄050051

出　　处：《肿瘤防治研究》2017年第4期257-261,共5页Cancer Research on Prevention and Treatment

摘　　要：目的检测食管胃交界部腺癌组织中O6-甲基鸟嘌呤-DNA甲基转移酶(O^6-methylguanineDNA methyltransferase,MGMT)基因启动子区甲基化状态以及蛋白表达情况,评价其与临床参数及预后的关系。方法选取107例食管胃交界部腺癌患者,应用甲基化特异性聚合酶链反应(methylmion specific PCR,MSP)检测MGMT基因甲基化情况,免疫组织化学法检测MGMT蛋白表达情况,分析两者与临床特征及生存时间的关系。结果癌组织中MGMT基因启动子区甲基化率显著高于癌旁正常组织;癌组织中MGMT蛋白阳性率显著低于癌旁正常组织。Spearman等级相关分析表明MGMT基因启动子区甲基化与MGMT蛋白表达呈负相关。MGMT基因启动子甲基化及蛋白表达与淋巴结转移、pTNM分期之间具有相关性。多因素Cox回归分析,MGMT基因启动子区甲基化、MGMT蛋白表达及pTNM分期是影响患者生存的独立预后因素。结论 MGMT基因启动子甲基化、MGMT蛋白表达与pTNM分期是影响食管胃交界部腺癌患者预后的独立影响因素。Objective To detect the methylation status of O^6-methylguanine-DNA methyltransferase（MGMT） promoter and its protein expression in adenocarcinoma of esophagogastric junction（AEJ） tissues. The relationship of MGMT methylation status and its protein expression with clinical features and survival were also analyzed. Methods The methylation status of MGMT promoter was detected by methylation specific polymerase chain reaction（MSP） in 107 AEJ cases. Immunohistochemistry staining was used to detect protein expression in the same group of specimen. Then, the relationship of MGMT methylation with clinical features and prognosis were analyzed. Results The promoter methylation rate of MGMT in cancerous specimen was higher than that in adjacent normal group. However, the MGMT expression rate was opposite. A correlation between promoter methylation of MGMT and protein expression were established by Spearman correlation analysis. MGMT promoter methylation and its protein expression had significant correlation with lymph node metastasis and pTNM stage. The Cox multivariate regression analysis indicated that MGMT promoter methylation, MGMT protein expression and pTNM stage were significant risk factors. Conclusion The promoter methylation of MGMT, MGMT protein expression and pTNM stage might be significant influence factors for 5 years survival time of AEJ patients.

关 键 词：食管胃交界部腺癌 MGMT基因 甲基化特异性聚合酶链反应 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]