PEDF对人脐静脉内皮细胞和肺癌SK-MES-1细胞增殖的影响及作用机制  

Effect and mechanism of PEDF on proliferation of human umbilical veinendothelial cells and lung cancer SK-MES-1 cells

作　　者：孟静[1] 束军[1] 李志斌[1] 

机构地区：[1]安徽医科大学第四附属医院呼吸内科,合肥230032

出　　处：《安徽医科大学学报》2017年第5期682-687,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金面上项目(编号:1308085MH141);安徽医科大学科研基金资助项目(编号:2010 xkj115)

摘　　要：目的探讨色素上皮衍生因子(PEDF)对SK-MES-1细胞和人脐静脉内皮细胞(HUVECs)增殖的影响及可能的机制。方法 CCK-8法检测不同浓度PEDF在不同作用时间条件下对HUVECs和SK-MES-1细胞增殖的影响;流式细胞仪检测不同浓度PEDF对此两种细胞凋亡的影响;qRTPCR检测PEDF对此两种细胞中血管内皮生长因子(VEGF)基因表达水平的影响。结果 CCK-8结果显示,PEDF对HUVECs和SK-MES-1细胞具有增殖抑制作用,呈一定浓度和时间依赖性(P<0.05);流式细胞术结果表明,实验组细胞的凋亡率高于对照组(P<0.05),高浓度用药组凋亡率高于低浓度用药组(P<0.05);qRT-PCR结果表明,与对照组相比,PEDF能抑制HUVECs和SK-MES-1细胞中VEGF mRNA水平的表达(P<0.05)。结论 PEDF的抗肿瘤作用主要包括抑制肿瘤血管生成和直接作用于肿瘤细胞两方面,PEDF对HUVECs和SK-MES-1细胞增殖的影响可能与降低VEGF表达水平有关。Objective To investigate the effect and potential mechanism of pigment epithelium derived factor （PEDF） acting upon SK-MES-1 cell and hmnan umbilical vein endothelial cells（HUVECs）. Methods CCK-8 was used to detect the effect of varying concentrations of PEDF upon HUVECs and SK-MES-1 cell, measuring the degree of cell proliferation and inhibition effect across varying times. The flow cytometry tests were carried out to invest gate the apoptosis of these two kinds of cells when exposed to varying concentration of PEDF. qRT-PCR were carried out to assess the vascular endothelial growth factor（VEGF） gene expression level in these two kinds of cells after treatment of PEDF. Results CCK-8 results revealed that PEDF had a concentration-dependent and time-de- pendent cell proliferation inhibition effect on SK-MES-1 cell and HUVECs（ P 〈 0.05 ） ; Flow cytometry showed that the apoptosis of the cells in the treatment group were higher than that of control group （ P 〈 0.05 ）, and the apopto- sis rate of high concentration group was higher than that of the low concentration group（P 〈 0.05） ; qRT-PCR re- suits showed that PEDF was able to inhibit expression of mRNA of VEGF in both HUVECs and SK-MES-1 cell com- pared with control samples（P 〈 0. 05 ）. Conclusion The antitumor properties of PEDF is mainly related to the in- hibition of tumor angiogenesis and direct effects on tumor ceils, the effect of PEDF on HUVECs and SK-MES-1 cell maybe related to the effects of PEDF on downregulating expression of VEGF.

关 键 词：色素上皮衍生因子 脐静脉内皮细胞 血管内皮生长因子 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]