P-糖蛋白在肝细胞癌鉴别诊断中的价值  

Potential value of P-glycoprotein in the differential diagnosis of hepatocellular carcinoma

作　　者：孙薇[1] 赵有财[2] 王劲松[2] 田智丹[2] 黄文斌[2] 赵建华[2] 

机构地区：[1]江苏建康职业学院病理学教研室,南京210029 [2]南京医科大学附属南京医院病理科,南京210006

出　　处：《诊断病理学杂志》2017年第4期274-277,共4页Chinese Journal of Diagnostic Pathology

基　　金：南京市医学科技发展项目(YKK11115)

摘　　要：目的观察P-糖蛋白(P-gp)在肝细胞癌和正常肝组织毛细胆管中的表达,探讨其在肝细胞癌鉴别诊断中的价值。方法应用免疫组化染色EnV ision法对36例不同分化程度的肝细胞癌组织和10例正常肝组织进行P-gp标记,观察其在毛细胆管中的表达情况。结果P-gp能清晰显示肝细胞癌及正常肝组织的毛细胆管结构。10例正常肝组织P-gp(+)(100%);36例肝细胞癌中29例(+)(80.6%),其中高、中、低分化肝细胞癌的阳性率分别为100%(7/7)、84.2%(16/19)和60%(6/10)。结论P-gp能特征性显示不同分化程度肝细胞癌的毛细胆管结构,可用于肝细胞癌的辅助诊断;其阳性率随肝细胞癌分化程度的降低而降低。Objective To investigate the application value of P-glycoprotein （P-gp） in the differential diagnosis of hepatocellular carcinoma （HCC） by immunohistoehemical （IHC） visualization of bile canaliculi. Methods 36 cases of HCC tissue （7 well differentiated, 19 moderate differentiated and 10 poor differentiated） and 10 cases of normal liver tissue were enrolled in our study. EnVision immunohistochemical examination was performed to detect the expression of P-gp and visualization of bile canaliculi. Results In the normal liver tissue group and the HCC group, a canalicular staining pattern was found for P-gpin in 10 specimens （100%, 10/10） and 29 specimens （80. 6%, 29/36）, respectively. Its positive rates were 100% （7/7）, 88.5% （23/26） and 60% （6/10） in well, moderately and poorly differentiated HCC, respectively. Conclusion P-gp may be one useful immunohistochemical marker in the differential diagnosis of HCC by exhibiting a characteristic canalicullar staining pattern. Its positive rate decreases from well to poorly differentiated HCC.

关 键 词：毛细胆管 P-糖蛋白 肝细胞癌 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤]