2株枯草芽孢杆菌的分离鉴定及特性研究  

Isolation,identification and characterization of two strains of Bacillus subtilis

作　　者：巴翠玉 张林波[1,2] 张培军[3] 赵福广[1,2] 李月红[1,2] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林农业大学生命科学学院,吉林长春130118 [3]吉林省卫生监测检验中心,吉林长春130000

出　　处：《华南农业大学学报》2017年第3期46-51,共6页Journal of South China Agricultural University

基　　金：国家自然科学基金(30972191);吉林省现代农业产业技术体系(201506);吉林省留学人员创新创业项目(201523)

摘　　要：【目的】寻求适合制作水产类微生态制剂的益生菌。【方法】从养鱼塘底泥和健康鲫鱼Carassius auratus肠道中分离芽孢杆菌,结合细菌形态学、生理生化特征和16S rRNA序列分析对其进行鉴定,对菌株安全性、高温耐受性、酸性耐受性、拮抗性及产酶情况等特性进行研究。【结果】分离到2株芽孢杆菌,分别命名为B1和B2,细菌形态学、生理生化特征和16S rRNA序列分析的结果显示,B1和B2均为枯草芽孢杆菌Bacillus subtilis。特性研究结果证实B1、B2菌株都具有较好的安全性、高温耐受性和酸性耐受性,可对致病性大肠埃希菌Escherichia coli、金黄色葡萄球菌Staphylococcus aureus、嗜水气单胞菌Aeromonas hydrophila以及温和气单胞菌A.sobria有良好的体外抑菌能力,均具有产脂肪酶、淀粉酶、蛋白酶和纤维素酶的能力。【结论】分离到2株性能优良的枯草芽孢杆菌,可将其作为水产微生态制剂的候选菌株。【Objective】 To explore potential probiotics in order to develop microecological agents for aquiculture.【Method】Bacillus stains were isolated from the pond sediment and healthy carp （Carassius auratus） intestine. The strains were identified by the morphological, physiological and biochemical characteristics and 16S rRNA sequence analysis. The safety, high temperature tolerance, pH tolerance, antagonistic effects and enzyme production of the strains were studied.【Result】Two Bacillus strains were isolated and named B1 and B2. They were identified as B. subtilis by morphology, physiological and biochemical characteristics and 16S rRNA sequence analysis. The B1 and B2 strains had good safety, high temperature tolerance and strong tolerance of acid. They had good antibacterial activity against pathogenic Escherichia coli, Staphylococcus aureus, Aeromonas hydrophila and A. sobria in vitro, and had the ability to produce lipase, amylase, protease and cellulase. 【Conclusion】Two B. subtilis strains were isolated, and both can be used as candidates for developing microecological agents for aquiculture.

关 键 词：枯草芽孢杆菌 分离鉴定 菌株特性 鲫鱼 16S RRNA 

分 类 号：S917.1[农业科学—水产科学]