调对前导增大的大鼠TMJ滑膜中COX-2、iNOS转归变化的研究  

Effect of Occlusal Intervention on the Expression of COX-2 and iNOS in the Temporomandibular Joint Synovial Membrane of Rat Caused by Increased Anterior Guidance

作　　者：李雨轩[1,2] 姚天华[1,3] 白乐康[2] 谭文宏[2] 韩会芳[2] 翟桐[3] 肖敏[1,2] 

机构地区：[1]陕西省颅颌面精准医学研究重点实验室,陕西西安710004 [2]西安交通大学口腔医院修复科,陕西西安710004 [3]西安交通大学口腔医院综合科,陕西西安710004

出　　处：《口腔医学研究》2017年第4期362-366,共5页Journal of Oral Science Research

基　　金：陕西省社会发展科技攻关基金项目(编号:2012k16-10-03)

摘　　要：目的:探讨调干预措施对前导增大引起的大鼠颞下颌关节(Temporomandibular joint,TMJ)滑膜中诱导型一氧化氮合成酶(Inducible nitric oxide synthase,iNOS)及环氧合酶-2(Cyclooxygenase-2,COX-2)表达变化的影响。方法:在大鼠上前牙粘结舌侧增大的金属冠,建立前导增大组(C),调组在建立前导斜度增大的基础上,分别于3、7、14、21d开始持续性调干预,建立I-3d、I-7d、I-14d、I-21d调组,所有大鼠第28天处死,取大鼠一侧TMJ进行苏木精.伊红(Hematoxylin-eosin,HE)染色、免疫组织化学(Immunohistochemistry,IHC)染色,另一侧TMJ滑膜组织进行实时定量聚合酶链反应(Real-time polymerase chain reaction,Real-time PCR)检测TMJ滑膜COX-2和iNOS的表达情况。结果:调组COX-2、iNOS mRNA表达较对照组明显减弱(P<0.05)。I-3d调组几乎不表达(P<0.005)。结论:早期调能减轻大鼠前导增大引起的滑膜炎症程度,同时降低其滑膜炎症因子COX-2、iNOS的表达。Objective：To investigate the effect of occlusal intervention on the expression of inducible nitric oxide synthase（iNOS）and cyclooxygenase-2（COX-2）in TMJ synovium of rats caused by increased anterior guidance.Methods：Metal crowns increased 30 degree on palatal side were cemented to the maxillary incisors of rat to build the model of anterior guidance increased group（C）.The intervention group was built by increased the anterior guidance of rat first,then the occlusal intervention respectively started on the day 3,7,14,and 21 to build the I-3d,I-7d,I-14 d,and I-21 dintervention group.All rats were sacrificed on day 28.TMJ joints were extracted by one side.HE staining was processed to observe the histological changes of TMJ synovium.IHC and real-time PCR analysis were evaluated to detect the levels of COX-2and iNOS in TMJ synovium.Results：The mRNA of iNOS and COX-2barely expressed in the intervention groups（I）compared with the anterior guidance increased group（C）（P〈0.05）.iNOS and COX-2 were scarcely expressed in the group I-3d.Conclusion：On the basis of increasing the anterior guidance inclination of rat,the early intervention would lead to much less expression of iNOS and COX-2 in TMJ synovium of rat.

关 键 词：前导斜度 调[牙合] 颞下颌关节滑膜 环氧合酶-2 一氧化氮合酶 

分 类 号：R782.6[医药卫生—口腔医学]