A型钾离子通道对人舌鳞癌Tca8113细胞增殖的影响  

Effects of A-type Potassium Ion Channels on the Proliferation of Human Tongue Squamous Cell Carcinoma Tca8113 Cells

作　　者：张晓艳 程俊[3] 许华丽 毛亮[3] 聂敏海 

机构地区：[1]西南医科大学附属口腔医院口腔内科,四川泸州646000 [2]西南医科大学口颌面修复重建和再生实验室,四川泸州646000 [3]西南医科大学电生理学教育部重点实验室,四川泸州646000

出　　处：《口腔医学研究》2017年第4期440-443,共4页Journal of Oral Science Research

基　　金：四川省应用基础研究计划项目(编号:LY-62)

摘　　要：目的:探究A型钾离子(Potassium,K^+)通道抑制后对舌鳞状细胞癌株(Tca8113)细胞的影响。方法:全细胞膜片钳技术记录和分析A型K^+通道阻滞剂4-氨基吡啶(4-AP)对Tca8113细胞K^+电流的影响;MTT检测A型K^+通道对细胞增殖的作用。结果:在终浓度为2 mmol/L 4-AP作用下,A型K^+电流的峰值从(267.04±13.84)pA降到(124.81±5.24)pA。在一定范围内,不同浓度4-AP能抑制Tca8113细胞在体外的增殖,且抑制程度随药物浓度和作用时间的增加而增强。结论:A型K^+通道参与Tca8113细胞增殖的过程,且A型K^+通道阻滞剂能抑制Tca8113细胞的增殖。Objective：To explore the effect of A-type potassium channels on the proliferation of Tca8113 cells.Methods：The whole cell patch clamp technique was used to record and analyze the inhibitory effect of 4-AP on A-type K＋current in Tca8113 cells,and MTT tests were used to test the inhibitory effect of 4-AP on cell proliferation in vitro.Results：Under the concentration of 2mmol/L 4-AP,the peak of A type K＋current was decreased from（267.04±13.84）pA to（124.81±5.24）pA.The different concentrations of 4-AP could inhibit the proliferation of Tca8113 cells,and the magnitude of inhibitive effect was concentrations and reaction time dependent.Conclusion：The A-type of K＋channels play a role in the process of Tca8113 cell proliferation,and the blocker of Atype K＋channel can inhibit the proliferation of Tca8113 cells.

关 键 词：舌肿瘤 鳞状细胞 4-氨基吡啶 钾通道 电压门控 细胞增殖 

分 类 号：R739.86[医药卫生—肿瘤]