电阻抗法快速测定巴氏杀菌牛乳中菌落总数  

Application research in rapid detection of aerobic plate count in pasteurized cow's milk by impedance method

作　　者：杜寒春[1] 叶开富[1] 刘绍刚[2] 黄岛平[1] 谭学才[2] 

机构地区：[1]广西壮族自治区分析测试研究中心,南宁530022 [2]广西民族大学化学化工学院,南宁530008

出　　处：《中国乳品工业》2017年第4期56-58,61,共4页China Dairy Industry

基　　金：广西直属公益性科研院所基本科研业务费专项项目(2011AC Z03);广西自然科学基金项目(2013GXNSFBA019048);广西科学研究与技术开发计划项目(桂科能14123006-24);国家自然科学基金项目(21367004;21667005)

摘　　要：建立了电阻抗法快速测定巴氏杀菌牛乳中菌落总数的方法。得到的阻抗检测时间与菌落总数的标准曲线方程为y=-0.5923t+9.4172,相关系数r=-0.9907,可信度高;其可信检测限为10~107m L-1,完全满足食品安全国家标准的要求;设立的样品评价标准可以快速、直观的对巴氏牛乳样品进行评价,在7.46 h内即可快速的判定样品是否合格,大大缩短了检测周期。结果表明,与国标法相比,电阻抗法简便快速、灵敏度高,又准确可靠,且适用于不同时期、来源及酸度的产品,具有广泛的适用性和实用性。A method for rapid detection of aerobic plate count in pasteurized cow＇s milk by impedance method was established. The follow- ing relationship between the germ count and the impedance detection time （t） was determined for the calibration equation： y=-0.5923 t＋ 9.4172, r=-0.9907,with high reliability, and the authentic detection threshold was 10～107 mL-1, fully meet the requirements of the national food safety standard. The establishment of the sample evaluation criteria could be fast, intuitive evaluation of the milk samples, the fastest 7.46 h could determine whether the sample is qualified, greatly shorten the detection time. Compared with the national standard method, the meth- od was simple, rapid, sensitive, accurate and reliable, and could be used in different periods, sources and acidity of the product, with a wide range of applicability and practicality.

关 键 词：电阻抗法 巴氏杀菌牛乳 菌落总数 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]