内生真菌HND5挥发性物质组分分析及其抑菌作用测定  

Identification and Fungistasis of Volatile Compounds Produced by Endophytic Fungi HND5

作　　者：陈奕鹏[1,2] 杨扬[2] 时涛[2] 蔡吉苗[2] 黄贵修[2] 

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所农业部热带作物有害生物综合治理重点实验室海南省热带农业有害生物监测与控制重点实验室,海南海口571101

出　　处：《热带作物学报》2017年第4期689-694,共6页Chinese Journal of Tropical Crops

基　　金：农业部现代农业人才支撑计划项目(No.0316001);中央级公益性科研院所基本科研业务费(No.2016hzs1J020)

摘　　要：HND5是从健康的臂形草叶片中分离得到的一株顶孢霉属(Acremonium sp.)内生真菌,可通过产生挥发性物质抑制病原菌生长。采用顶空固相微萃取技术(HS-SPME)提取富集了内生真菌HND5的挥发性有机物(VOCs),利用气相色谱-质谱联用法(GC-MS)进行了挥发性物质的组分分析,共分离鉴定得到14个单体化合物。活性分析发现,石竹烯(Caryophyllene)和4-乙烯基-1,2-二甲氧基苯(Benzene,4-ethenyl-1,2-dimethoxy-)具有抑菌活性,对峙试验表明2种物质均对尖孢镰刀菌(Fusarium oxysporum)、胶胞炭疽菌(Colletotrichum gloeosporioides)和多主棒孢菌(Corynespora cassiicola)具良好的抑制作用,其中石竹烯的有效中浓度(EC50)分别为2 916.07、729.54和404.23μL/L,4-乙烯基-1,2二甲氧基苯的有效中浓度(EC50)分别为62.72、27.60和23.54μL/L。Endophytic fungi HND5 which was isolated from healthy leaves of Brachiaria sp. could inhibit many plant pathogens with volatile substances. In this article, solid phase micro-extraction （SPME） was used to collect volatile organic compounds （VOCs） produced by HND5 from the head space. And GC-MS was used to analyzed /he volatile compounds of HND5. At last, 14 compounds were identified from VOCs produced by HND5. Within these compounds, Caryophyllene and Benzene, 4-ethenyl-l,2-dimethoxy- were found antagonistic against growth of Fusarium oxysporum, Colletotrichum gloeosporioides and Corynespora cassiicola. ECso values of Caryophyllene were 2 916.07, 729.54 and 404.23 μL/L, respectively. And ECso values of Benzene, 4-ethenyl-l,2-dimethoxy were 62.72, 27.60 and 23.54μL/L, respectively.

关 键 词：内生真菌 挥发性有机物 气相色谱-质谱联用 抑菌作用 

分 类 号：S432.1[农业科学—植物病理学]