丙戊酸对HL-60/HT细胞P27^(Kip1)、P170表达水平的影响  

Impact of valproic acid to expression level of P27^(Kip1) and P170 of HL-60/HT cells

作　　者：胡建锋 尹松梅[2] 谢双锋[2] 聂大年[2] 李益清[2] 

机构地区：[1]广东省佛山市顺德区中医院肿瘤科,广东佛山528333 [2]中山大学孙逸仙纪念医院血液科,广东广州510120

出　　处：《中国医药科学》2017年第6期151-153,161,共4页China Medicine And Pharmacy

摘　　要：目的了解丙戊酸对HL-60/HT细胞P27^(Kip1)的影响。方法建立白血病耐药细胞株HL-60/HT,MTT法检查丙戊酸作用前后细胞增殖情况及对Ara-c耐药性的改变,流式细胞仪检测HL-60、HL-60/HT细胞P27^(Kip1)、P170的表达。结果 VPA能增加HL-60、HL-60/HT细胞P27^(Kip1)的表达水平(P<0.05),而不影响P170的表达(P>0.05);Ara-c不能影响HL-60、HL-60/HT细胞的P27^(Kip1)、P170的表达(P>0.05);VPA与Ara-C联用能增加HL-60、HL-60/HT细胞P27^(Kip1)的表达水平(P<0.05),不影响P170的表达(P>0.05)。结论 HL-60/HT细胞P27^(Kip1)低于HL-60,丙戊酸能增加HL-60/HT细胞P27^(Kip1)的表达。Objective To understand the impact of valproie acid to expression level of P27^Kip1 and P170 of HL- 60/HT cells. Methods Leukemia resistant cell line HL-60/HT was established. MIT method was used to deteeeell proliferation before and after valproie acid and its changes to drug resistance of Ara-c. Flow eytometry was used to detect the expression of P27^Kip1 and PI70 of HL-60 and HL-60/HT ceils, Results VPA could increase the expression level of P27^Kip1 of HL-60 and HL-60/HT eells（P 〈 0.05）. It did afloat the expression of P170（p 〉 0,05）. Ara-e could not affect the expression level of P27^Kip1 of HL-60 and HL-60/HT eells（P 〈 0.05）. VPA combined with Ara-C can increase the expression level of P27^Kip1 of HL-60 and HL-60/HT cells（P 〈 0.05）. It did affect the expression of P170（P 〉 0.05）. Conclusion P27^Kip1 of HL-60/HT cells is lower than HL-60 cells. Valproie acid can the expression of P27^Kip1 of HL-60/HT cells.

关 键 词：白血病 丙戊酸 HL-60/HT细胞 耐药性 

分 类 号：R733.7[医药卫生—肿瘤]