动物双歧杆菌AD011表面脂蛋白的全基因组预测和功能分析  

Genome-wide Prediction and Functional Analysis of Surface Lipoproteins of Bifidobacterium animalis AD011

作　　者：栗金柳 王茗悦 朱德全[2] 高峰[1] 于陆雪子 周金影[1] 郝莹莹[1] 张宛麟 周博[3] 

机构地区：[1]佳木斯大学生命科学学院,黑龙江佳木斯154007 [2]佳木斯大学农业与环境生物技术研究所,黑龙江佳木斯154007 [3]佳木斯大学信息电子技术学院,黑龙江佳木斯154007

出　　处：《安徽农业科学》2017年第11期117-118,共2页Journal of Anhui Agricultural Sciences

基　　金：黑龙江大学生创新创业项目(201610222059);佳木斯大学大学生科技创新项目(xsyz2016-02);黑龙江省卫生计生委科研项目(2016-325)

摘　　要：[目的]运用生物信息学方法预测动物双歧杆菌AD011定位于细胞膜的表面脂蛋白,为后续研究该菌与宿主相互作用的分子机制提供基础资料。[方法]用Signal P 4.0和Lipo P软件对动物双歧杆菌AD011全基因组编码蛋白中表面脂蛋白和信号肽进行预测,同时采用COG功能数据库对预测的脂蛋白进行功能注释和聚类分析。[结果]对动物双歧杆菌AD011基因组1 518个蛋白的表面脂蛋白预测,结果表明,19个蛋白具有脂蛋白信号肽。功能分析显示,该菌株19个脂蛋白中有3个蛋白没有功能注释,16个蛋白有功能注释,其中10个蛋白参与氨基酸代谢与转运、3个蛋白参与碳水化合物代谢与转运,3个蛋白参与无机盐离子代谢与转运。[结论]动物双歧杆菌AD011表面脂蛋白参与的功能主要与营养物质的转运和代谢有关。[ Objective ] The surface lipoproteins of Bifidobacterium animalis AD011 were predicted by bioinformatics method, which provided the basis for interaction molecular mechanisms between bacteria and their host. [ Method] SignalP 4.0 and LipoP softwares were used to analyze the lipoproteins and characteristic of signal peptides. Function of lipoproteins was also analyzed by COG database. [ Result] There were 19 proteins which possessed signal peptide of lipoproteins from 1 518 proteins coded by B. animalis ADOll genome. Function analysis revealed that the strain contains 3 hypothetical proteins without function annonation. 16 proteins have function annonation which mainly involved in ami- no acid metabolism and transport（ 10 proteins）, transport of carbohydrate metabolism （3 proteins）, metabolism of inorganic ion transport（ 3 proteins）. [ Conclusion ] Function of surface lipoproteins of B. animalis ADO11 are mainly associated with the transport and metabolism of nutrients.

关 键 词：动物双歧杆菌 基因组 脂蛋白 信号肽 

分 类 号：TS201.3[轻工技术与工程—食品科学]