京尼平苷酸对大鼠缺血性脑损伤神经保护作用  

Study of Neuroprotection of Geniposidic Acid on Ischemic Brain Injury of Rat

作　　者：黄嘉驹 严莉[1] 欧阳昌汉[1] 

机构地区：[1]湖北科技学院药学院,湖北咸宁437100 [2]武汉市中心医院药学部

出　　处：《湖北科技学院学报（医学版）》2017年第2期93-96,103,88,共6页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北省卫计委面上项目(WJ2017M248);国家级创新训练项目(201410927002)

摘　　要：目的观察口服京尼平苷酸对缺血性脑损伤大鼠的保护作用。方法 SD大鼠随机分成四组,假手术组、脑缺血模型组、京尼平苷酸组和安理申对照组,每组10只。采用Morris水迷宫实验评价大鼠学习记忆能力;HE染色观察大鼠海马CA1区、皮质内锥体层、扣带回皮质层和皮质外颗粒层的神经元数量的变化。结果水迷宫结果显示假手术组和脑缺血模型组大鼠平均逃避潜伏期分别为(31.81±9.51)s、(87.21±12.18)s;京尼平苷酸组大鼠平均逃避潜伏期为(45.48±15.04)s比脑缺血模型组明显缩短(P<0.01)。HE染色结果脑缺血模型组相对于假手术组各脑区神经元总数显著降低,变性神经元数和变性率显著增加(P<0.01)。京尼平苷酸组与脑缺血模型组相比较,CA1区、扣带回区、外颗粒层及内锥体层4个脑区的神经元总数均显著增加,变性神经元数及变性率均显著降低(P<0.01)。京尼平苷酸组与安理申对照组比较,除在CA1区神经元总数方面前者高于后者(P<0.01)以外,其余各脑区指标的差异均无差异(P>0.05)。结论京尼平苷酸可以改善大鼠缺血性脑损伤,能减少缺血性脑损伤大鼠大脑皮质和海马神经元的缺血性坏死。Objective To study the protective effect of oral administration of geniposidic acid on ische-mic brain injury in rats. Methods A total of 40 SD rats were randomly divided into one of four groups in equal numbers （n = 10） ： sham operation group,cerebral ischemia model group,geniposidic acid group and Anli Shen group. Morris water maze test was used to evaluate the learning and memory ability of rats. HE staining was used to observe the changes of neuronal cells in hippocampal CA1 region, cortical pyramidal layer, cingulate cortex and extracorporeal granule layer. Results Morris water maze showed that the mean escape latency was （31.81 ±9.51）s in sham operation group and （87. 21 ±12. 18）s in model group. The mean escape latency of the geniposidic acid group was （45.48 ± 15. 04） s and significantly shorter than that of the model group（P 〈 0. 01）. The total number of neurons in the brain of the HE staining model was significantly decreased, and the number of degenerated neurons and degeneration rate was increased significantly （ P 〈0. 01）. Compared with the model group,the geniposidic acid group number of neurons in the CA1 region,the cingulate zone,the outer granular layer and the inner cone layer significantly increased, and the number of deformed neurons and degen-eration rate were decreased significantly （ P 〈0. 01）. There was no significant difference between the other groups（P 〉 0.05）. Conclusion Geniposidic acid can improve ischemic brain injury in rats and reduce ische-mic necrosis of cerebral cortex and hippocampal neurons in rats with ischemic brain injury.

关 键 词：京尼平苷酸 慢性脑缺血 大脑皮质 神经保护 

分 类 号：R-332[医药卫生]